J425
425 .
A1 MARINE MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J425 #
ml
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Poorly crystalline iron(III) oxide | 100.00 | mM | - | - | |
| NaCl | 20.00 | g | 400 | 6844.63 | |
| KCl | 0.77 | g | 15.4 | 206.573 | |
| NH4Cl | 0.25 | g | 5 | 93.475 | |
| KH2PO4 | 0.10 | g | 2 | 14.697 | |
| MgSO4 x 7 H2O | 0.20 | g | 4 | 16.229 | |
| Trace vitamins | 10.00 | ml | - | - | |
| Trace mineral solution | 10.00 | ml | - | - | |
| Acetate solution 41.0 g/L | 10.00 | ml | - | - | |
| APM salts solution | 20.00 | ml | - | - | |
| 1 Add components except acetate and APM salts solutions to distilled water, bring volume to 1.0 L and adjust pH to 6.8. Distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, sparge with the same gas mixture for 10 - 15 min, seal with butyl rubber stoppers and autoclave. Separately autoclave acetate and APM salts solutions under a N2 gas atmosphere and add to the medium anaerobically. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Sodium acetate | 41 | g | 41 | 499.817 |
| Distilled water | 1000 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| MgCl2 x 6 H2O | 21.20 | g | 21.2 | 104.278 |
| CaCl2 x 2 H2O | 3.04 | g | 3.04 | 20.678 |
| Distilled water | 1000.00 | ml | - | - |
Solution occurs in 3
other media (first defined in Medium J411).
Equipment needed: Centrifuge
| 1 Dissolve 108 g FeCl3 in 1.0 L distilled water. Adjust pH to 7.0 with 10 N NaOH. Allow the suspension to sit for 30 min, then readjust pH to 7.0. Centrifuge the suspension at 5,000 x g for 20 min and pour off supernatant. Wash the precipitate with distilled water by resuspending in distilled water followed by the centrifugation. Repeat the washing at least 5 times, then, resuspend in 1.0 L distilled water. Use the Fe(III) suspension as 0.67 M solution. More precisely, check the concentration of Cl- to be less than 1 mM and determine the concentration of Fe(III) by using hydroxylamine as described by Lovley and Phillips (Appl. Environ. Microbiol. 54, 751-757, 1986). |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Solution occurs in 4
other media (first defined in Medium J411).
| 1 Use Trace minerals of Medium No. 151 supplemented with 0.025 g (final) Na2MoO4 x 2H2O, 0.024 g NiCl2 x 6H2O and 0.025 g Na2WO4 x 2H2O (per liter). | |||||
| 2 Comment: For strain JCM 12378, inoculate with a 10% inoculum. |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 6.8 |
| Equipment needed: | Anaerobic workbench, Autoclave, Centrifuge |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 400 | 6844.63 |
| KCl | 15.4 | 206.573 |
| MgCl2 x 6 H2O | 8.48 | 41.711 |
| Sodium acetate | 8.2 | 99.963 |
| NH4Cl | 5 | 93.475 |
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