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J430. DESULFOSPOROMUSA MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.00 g 2.97 20.911
2 0.20 g 0.198 1.455
3 0.30 g 0.297 5.553
4 1.00 g 0.99 16.941
5 0.40 g 0.396 1.948
6 0.50 g 0.495 6.64
7 0.15 g 0.149 1.01
8 FeCl2 solution 1.00 ml - -
9 Trace element solution 1.00 ml - -
10 1.00 mg 9.9e-4 0.004
11 900.00 ml 889.491 49374.2
12
(*, 1.0 M)
20.00 ml - -
13
(10%)
10.00 ml - -
14 Trace vitamins
(*)
10.00 ml - -
15
(*, 8%)
60.00 ml 10.261 122.14
16
(5%)
8.00 ml - -
Instructions:
  1. Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): #12-16
  2. Adjust pH to 7.1 - 7.4, if necessary.
Solution occurs in 89 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 70 mg 0.07 0.514
2 100 mg 0.1 -
3 6 mg 0.006 0.097
4 190 mg 0.19 -
5 2 mg 0.002 -
6 24 mg 0.024 -
7 36 mg 0.036 0.149
8 1000 ml 998.207 55408.8
Solution occurs in 87 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(7.7 M, 25%)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 990.0 ml 988.225 54854.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 901.341 50032
NaHCO3 10.261 122.14
Na2SO4 2.97 20.911
NaCl 0.99 16.941
KCl 0.495 6.64
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