JCM 431 . BN3 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Basal medium 1000.00 ml - -
Trace element solution 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Trace vitamins 10.00 ml - -
Vitamin B12 5.00 �g - -
Glucose 3.60 g 3.557 19.745
Yeast extract
(BD-Difco)
0.50 g 0.494 -
Tryptone
(BD-Difco)
1.50 g 1.482 20.853
Sulfur
(powder)
20.00 g 19.763 616.241
NaHCO3 3.40 g 3.36 39.993
Resazurin 0.50 mg 4.9e-4 0.002
Na2S x 9 H2O 0.36 g 0.356 1.481
1 Autoclave Basal medium supplemented with Trace element solution, Selenite-tungstate solution and resazurin under N2-CO2 (4:1, v/v). Separately autoclave glucose, yeast extract and tryptone (10% each) and add to the medium. Filter-sterilize Trace vitamins and NaHCO3 (8%) solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels containing sulfur under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Before inoculation, reduce the medium with 5% Na2S x 9H2O solution (autoclaved under a N2 atmosphere). Readjust pH to 7.2, if necessary.
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.000 g 1 17.112
MgCl2 x 6 H2O 0.400 g 0.4 1.968
CaCl2 x 2 H2O 0.075 g 0.075 0.51
NH4Cl 0.250 g 0.25 4.674
KH2PO4 0.200 g 0.2 1.47
KCl 0.500 g 0.5 6.707
Distilled water 1000.000 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 987.0 ml - -
HCl
(25%)
12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulfur 19.763 616.241
Glucose 3.557 19.745
NaHCO3 3.36 39.993
Tryptone 1.482 20.853
NaCl 0.988 16.909
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain
temperature thermophilic 1 strain