Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Basal medium | 1000.00 | ml | ||
|
Trace element solution | 1.00 | ml | ||
|
Selenite-tungstate solution | 1.00 | ml | ||
|
Trace vitamins | 10.00 | ml | ||
|
Vitamin B12 | 5.00 | �g | ||
|
Glucose | 3.60 | g | ||
|
Yeast extract
(BD-Difco) |
0.50 | g | ||
|
Tryptone
(BD-Difco) |
1.50 | g | ||
|
Sulfur
(powder) |
20.00 | g | ||
|
NaHCO3 | 3.40 | g | ||
|
Resazurin | 0.50 | mg | ||
|
Na2S x 9 H2O | 0.36 | g | ||
1 Autoclave Basal medium supplemented with Trace element solution, Selenite-tungstate solution and resazurin under N2-CO2 (4:1, v/v). Separately autoclave glucose, yeast extract and tryptone (10% each) and add to the medium. Filter-sterilize Trace vitamins and NaHCO3 (8%) solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels containing sulfur under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Before inoculation, reduce the medium with 5% Na2S x 9H2O solution (autoclaved under a N2 atmosphere). Readjust pH to 7.2, if necessary. |
Compound | Amount | Unit | |
---|---|---|---|
|
NaCl | 1.000 | g |
|
MgCl2 x 6 H2O | 0.400 | g |
|
CaCl2 x 2 H2O | 0.075 | g |
|
NH4Cl | 0.250 | g |
|
KH2PO4 | 0.200 | g |
|
KCl | 0.500 | g |
|
Distilled water | 1000.000 | ml |
Compound | Amount | Unit | |
---|---|---|---|
|
Distilled water | 987.0 | ml |
|
HCl
(25%) |
12.5 | ml |
|
FeSO4 x 7 H2O | 2.1 | g |
|
H3BO3 | 30.0 | mg |
|
MnCl2 x 4 H2O | 100.0 | mg |
|
CoCl2 x 6 H2O | 190.0 | mg |
|
NiCl2 x 6 H2O | 24.0 | mg |
|
CuCl2 x 2 H2O | 2.0 | mg |
|
ZnSO4 x 7 H2O | 144.0 | mg |
|
Na2MoO4 x 2 H2O | 36.0 | mg |
Compound | Amount | Unit | |
---|---|---|---|
|
NaOH | 0.4 | g |
|
Na2SeO3 x 5 H2O | 6.0 | mg |
|
Na2WO4 x 2 H2O | 8.0 | mg |
|
Distilled water | 1000.0 | ml |
Compound | Amount | Unit | |
---|---|---|---|
|
Biotin | 2.0 | mg |
|
Folic acid | 2.0 | mg |
|
Pyridoxine hydrochloride | 10.0 | mg |
|
Thiamine HCl | 5.0 | mg |
|
Riboflavin | 5.0 | mg |
|
Nicotinic acid | 5.0 | mg |
|
Calcium pantothenate | 5.0 | mg |
|
Vitamin B12 | 0.1 | mg |
|
p-Aminobenzoic acid | 5.0 | mg |
|
Lipoic acid | 5.0 | mg |
|
Distilled water | 1000.0 | ml |