Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 1.00 | g | 500 | 8555.78 | |
MgCl2 x 6 H2O | 0.10 | g | 50 | 245.938 | |
CaCl2 x 2 H2O | 0.05 | g | 25 | 170.051 | |
NH4Cl | 0.25 | g | 125 | 2336.89 | |
KH2PO4 | 0.10 | g | 50 | 367.415 | |
KCl | 0.50 | g | 250 | 3353.45 | |
Yeast extract | 0.50 | g | 250 | - | |
CAPSO | 11.86 | g | 5930 | - | |
Na2SO4 | 4.00 | g | 2000 | 14080.5 | |
Trace element solution
(see Medium No. 294) |
1.00 | ml | - | - | |
Selenite-tungstate solution | 1.00 | ml | - | - | |
Sodium lactate | 2.24 | g | 1120 | 9994.65 | |
Resazurin | 1.00 | mg | 0.5 | 2.182 | |
Sodium dithionite | 0.10 | g | 50 | 287.175 | |
1 Dissolve components except sodium lactate and sodium dithionite in 900 ml distilled water and adjust pH to 9.0 with NaOH. Bring to a boil, and cool down under a N2 gas stream. Distribute the medium into culture vessels (e.g., 9 ml of the medium in Hungate tubes) under N2, seal with butyl rubber stoppers and autoclave. Separately autoclave 0.2 M sodium lactate under a N2 atmosphere. After cooling, add 1/9 volume of the sodium lactate solution to the medium. Readjust pH to 9.0, if necessary. Prior to use, reduce the medium with a freshly prepared, filter-sterilize 1% sodium dithionite solution. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaOH | 0.4 | g | 0.4 | 10.001 |
Na2SeO3 x 5 H2O | 6.0 | mg | 0.006 | 0.023 |
Na2WO4 x 2 H2O | 8.0 | mg | 0.008 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 9.0 |
Equipment needed: | Autoclave, Filter, Hungate tubes |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
CAPSO | 5930 | - |
Na2SO4 | 2000 | 14080.5 |
Sodium lactate | 1120 | 9994.65 |
NaCl | 500 | 8555.78 |
KCl | 250 | 3353.45 |
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