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JCM 454 . SPIROCHAETA CALIFORNIENSIS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J454 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 10000 171116
Na2CO3 2.76 g 920 8680.23
NaHCO3 24.00 g 8000 95230.2
KCl 0.20 g 66.667 894.254
K2HPO4 0.20 g 66.667 382.755
MgCl2 x 6 H2O 0.10 g 33.333 163.959
NH4Cl 1.00 g 333.333 6231.69
Na2S x 9 H2O 0.40 g 133.333 555.135
Yeast extract 0.50 g 166.667 -
Glucose 5.00 g 1666.67 9251.04
Trace vitamins 2.00 ml - -
Trace minerals solution 1.00 ml - -
Resazurin 1.00 mg 0.333 1.454
1 Dissolve components except Na2CO3, NaHCO3 and Na2S x 9H2O in 700 ml distilled water and autoclave under a N2 atmosphere in a bottle capped with a butyl rubber stopper. Separately dissolve Na2CO3 and NaHCO3 in 300 ml distilled water, filter-sterilize, and add to the medium aseptically and anaerobically. Adjust pH to 9.0 - 9.5, if necessary. Prior to inoculation, reduce the medium by the addition of a 5% Na2S x 9H2O solution autoclaved under a N2 atmosphere.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace minerals solution #

1000
Solution occurs in 5 other media (first defined in Medium J228).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
Fe(NH4)2(SO4)2 x 6 H2O 0.200 g 0.2 -
Na2SeO3 0.100 g 0.1 -
Na2MoO4 x 2 H2O 0.100 g 0.1 0.413
Na2WO4 x 2 H2O 1.000 g 1 3.032
MnSO4 x n H2O 0.100 g 0.1 -
ZnSO4 x 7 H2O 0.100 g 0.1 0.348
NiCl2 x 6 H2O 0.025 g 0.025 0.193
CuSO4 x 5 H2O 0.010 g 0.01 0.04
Distilled water 1000.000 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals.
2 Adjust final pH to 7.0.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.2
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 10000 171116
NaHCO3 8000 95230.2
Glucose 1666.67 9251.04
Na2CO3 920 8680.23
NH4Cl 333.333 6231.69
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Cultivation metadata from 2 strains BacDive

pH 6 - 8 1 strain
pH 10 - 12 1 strain
phyla 2 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains