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J462. SALTWATER MEDIUM WITH LACTATE

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 20.00 g 3333.33 57036.1
2 4.00 g 666.667 4693.41
3 3.00 g 500 2459.42
4 0.15 g 25 170.056
5 0.75 g 125 2336.83
6 0.60 g 100 734.835
7 0.09 g 15 126.048
8 0.50 g 83.333 1117.8
9 2.50 g 416.667 4959.93
10 Trace element solution 1.00 ml - -
11 Vitamin solution 1.00 ml - -
12 Thiamine solution 1.00 ml - -
13 Vitamin B12 solution 1.00 ml - -
14 Riboflavin solution 1.00 ml - -
15 Selenite-tungstate solution 1.00 ml - -
16 2.25 g 375 -
17 0.30 g 50 208.169
18 1.00 mg 0.167 0.727
Instructions:
  1. Dissolve components except NaHCO3, Vitamin solution, Thiamine solution, Vitamin B12 solution, sodium lactate and Na2S x 9H2O in 900 ml distilled water and adjust pH to 7.0. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels (e.g., 9 ml of the medium in Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Separately autoclave 0.2 M sodium lactate under a N2 atmosphere. After cooling, add 1/9 volume of the sodium lactate solution, and filter-sterilized 8% NaHCO3 solution, Vitamin solution, Thiamine solution and Vitamin B12 solution to the medium. Readjust pH to 7.0, if necessary. Prior to use, reduce the medium with Na2S x 9H2O (3% solution, autoclaved and stocked under N2).
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.5 mg 0.025 0.066
2 100.0 ml 998.207 55408.8
Solution occurs in 38 other media (first defined in medium J439 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(25%)
12.5 ml 3.963 108.679
2 2.1 g 2.1 7.553
3 30.0 mg 0.03 0.485
4 100.0 mg 0.1 -
5 190.0 mg 0.19 -
6 24.0 mg 0.024 -
7 2.0 mg 0.002 -
8 144.0 mg 0.144 -
9 36.0 mg 0.036 0.149
10 987.0 ml 985.23 54688.5
Solution occurs in 14 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4 mg 0.04 0.292
2 1 mg 0.01 0.041
3 10 mg 0.1 0.812
4 5 mg 0.05 -
5 10 mg 0.1 -
6
(10 mM, pH 7.1)
100 ml - -
Solution occurs in 69 other media (first defined in medium J431 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 0.4 10.001
2 6.0 mg 0.006 -
3 8.0 mg 0.008 -
4 1000.0 ml 998.207 55408.8
Solution occurs in 10 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10 mg 0.1 -
2
(25 mM, pH 3.4)
100 ml - -
Solution occurs in 18 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 mg 0.05 0.032
2 100 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 3333.33 57036.1
Na2SO4 666.667 4693.41
Distilled water 663.308 36819.1
MgCl2 x 6H2O 500 2459.42
NaHCO3 416.667 4959.93
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