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J470. VULCANIBACILLUS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(Sigma)
30.00 g 29.354 -
2 1.00 g 0.978 11.512
3 Wolfe's mineral elixir 2.00 ml - -
4 0.05 mg 4.9e-5 -
5 1.00 g 0.978 -
6 0.50 mg 4.9e-4 0.002
7 0.10 g 0.098 0.352
8 1.00 g 0.978 11.648
9 0.50 g 0.489 -
10 Trace vitamins 20.00 ml - -
11 1000.00 ml 976.719 54216.1
Instructions:
  1. Dissolve ingredients except FeSO4 x 7H2O, NaHCO3, yeast extract and vitamins, and adjust pH to 7.0. Bring to a boil for 3 min, then cool to room temperature under a N2 gas stream. Dispense the medium under the same gas atmosphere into culture vessels, seal with butyl rubber stoppers and autoclave. Add sterile anaerobic stock solutions of FeSO4 x 7H2O (1%, in 0.1 N H2SO4), NaHCO3 (8%, under a N2 x CO2 gas mixture), yeast extract (10%) and vitamins (filter-sterilized) to the medium. Adjust final pH of the medium to 7.0 - 7.2. Add 10 - 20 mg sodium dithionite per liter [e.g., from 5%(w/v) solution, freshly prepared under N2 and filter-sterilized], if the medium is not completely reduced after inoculation.
Solution occurs in 8 other media (first defined in medium J470 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 30.00 g 30 121.714
2 5.00 g 5 -
3 10.00 g 10 171.108
4 1.00 g 1 3.597
5 1.80 g 1.8 -
6 1.00 g 1 6.802
7 1.80 g 1.8 -
8 0.10 g 0.1 -
9 0.18 g 0.18 -
10 0.10 g 0.1 1.617
11 0.10 g 0.1 0.413
12 2.80 g 2.8 -
13 0.10 g 0.1 -
14 0.10 g 0.1 0.529
15 1000.00 ml 998.207 55408.8
Instructions:
  1. First adjust pH to 1.0 with diluted H2SO4, then dissolve the salts.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.9
Sea salts 29.354 -
NaHCO3 0.978 11.648
NaNO3 0.978 11.512
Sodium pyruvate 0.978 -
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