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J484. MODIFIED METHANOCALDOCOCCUS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.14 g 0.137 0.784
2 0.14 g 0.137 0.929
3 0.25 g 0.244 4.56
4 3.40 g 3.317 13.458
5 2.70 g 2.634 12.957
6 0.33 g 0.322 4.319
7 30.00 g 29.268 500.805
8 Iron stock solution 5.00 ml - -
9 Trace minerals solution 10.00 ml - -
10 Trace vitamins 10.00 ml - -
11 1.00 g 0.976 -
12
(BD-Difco)
2.00 g 1.951 -
13 0.40 g 0.39 -
14 1.00 mg 9.8e-4 0.004
15 1000.00 ml 973.86 54057.4
Instructions:
  1. Mix ingredients except Na2S2O3 x 5H2O, yeast extract, Trace vitamins and 2-mercaptoethanol, and adjust pH to 6.0. Bring to a boil for 5-10 sec, then, cool down under a stream of H2-CO2 (80:20, v/v). Dispense the medium into suitable culture vessels under a stream of H2-CO2 (80:20, v/v), seal with butyl rubber stoppers and autoclave 115C for 40 min. Separately autoclave Na2S2O3 x 5H2O and 2-mercaptoethanol as 50 g/L solutions and yeast extract as 100 g/L solution under a N2 atmosphere. Filter-sterilize Trace vitamins and store under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the Na2S2O3 x 5H2O, yeast extract, Trace vitamins and 2-mercaptoethanol solutions to the medium. Pressurize the inoculated bottles to 200 kPa H2-CO2 (80:20, v/v).
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.500 g 1.5 7.848
2 3.000 g 3 12.171
3 0.500 g 0.5 -
4 1.000 g 1 17.111
5 0.100 g 0.1 0.36
6 0.100 g 0.1 -
7 0.100 g 0.1 0.68
8 0.100 g 0.1 -
9 0.010 g 0.01 -
10 0.010 g 0.01 0.039
11 0.010 g 0.01 0.162
12 0.010 g 0.01 0.041
13 0.025 g 0.025 -
14 0.300 mg 3.0e-4 -
15 1000.000 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 1 other media (first defined in medium J228 )
Instructions:
  1. Dissolve 0.2 g of Fe(NH4)2(SO4)2 x 6H2O in 5 ml of distilled water containing 2 drops of concentrated HCl. When the Fe(NH4)2(SO4)2 x 6H2O crystal have dissolved, bring volume up to 100 ml.

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 993.337 55138.5
NaCl 29.278 500.972
MgSO4 x 7H2O 3.346 13.577
MgCl2 x 6H2O 2.634 12.957
Yeast extract 1.951 -
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