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JCM 501 . MODIFIED MARINE MEDIUM FOR DETHIOSULFOBACTER

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Solution A #

765
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 26.144 447.361
MgCl2 x 6 H2O 3.00 g 3.922 19.289
Na2SO4 4.00 g 5.229 36.812
KH2PO4 0.20 g 0.261 1.921
KCl 0.50 g 0.654 8.767
NH4Cl 0.25 g 0.327 6.11
CaCl2 x 2 H2O 0.15 g 0.196 1.334
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Yeast extract 0.50 g 0.654 -
Vitamin solution 1.00 ml - -
Vitamin B12
(Medium No. 403) 		1.00 ml - -
Distilled water 760.00 ml - -
1 Mix components except Vitamin and Vitamin B12 solutions, adjust pH to 7.0 and autoclave under a N2 gas atmosphere. After autoclaving, add filter-sterilized Vitamin and Vitamin B12 solutions.

Solution B #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sodium glutamate monohydrate 1.87 g 18.7 99.931
Distilled water 100.00 ml - -

Solution C #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 5 g 50 201.462
Distilled water 100 ml - -

Solution D #

30
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 2.5 g 83.333 991.981
Distilled water 30.0 ml - -
1 Filter-sterilize and store under a N2-CO2 (4:1, v/v) gas mixture.

Solution E #

10
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sodium dithionite 20 mg 2 11.487
Distilled water 10 ml - -
1 Mix components and filter-sterilize just before use.
2 Separately sterilize the above described solutions. Mix Solutions A, B, C and D, distribute into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Then, add Solution E and adjust pH to 7.2 - 7.8, if necessary.

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Vitamin solution #

100
Solution occurs in 14 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
p-Aminobenzoic acid 4 mg 0.04 0.292
Biotin 1 mg 0.01 0.041
Nicotinic acid 10 mg 0.1 0.812
DL-Calcium pantothenate 5 mg 0.05 0.105
Pyridoxine hydrochloride 10 mg 0.1 -
Sodium phosphate buffer
(10 mM, pH 7.1) 		100 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
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