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JCM 508 . MODIFIED MMJS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J508 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 19.782 338.508
K2HPO4 0.09 g 0.089 0.511
KH2PO4 0.07 g 0.069 0.509
CaCl2 x 2 H2O 0.80 g 0.791 5.382
NH4Cl 0.25 g 0.247 4.623
NaNO3 1.00 g 0.989 11.637
MgSO4 x 7 H2O 4.00 g 3.956 16.052
MgCl2 x 6 H2O 3.00 g 2.967 14.596
KCl 0.33 g 0.326 4.378
NiCl2 x 6 H2O 1.00 mg 9.9e-4 0.008
Na2SeO3 x 5 H2O 1.00 mg 9.9e-4 0.004
Fe2(SO4)3 x n H2O 0.01 g 0.01 -
H2WO4 1.00 mg 9.9e-4 0.004
Na2S2O3 x 5 H2O 2.00 g 1.978 7.971
Sulfur
(powder) 		3.00 g 2.967 92.528
Trace mineral solution 10.00 ml - -
Trace vitamins 1.00 ml - -
NaHCO3 2.00 g 1.978 23.549
Distilled water 1000.00 ml - -
1 Mix ingredients except sulfur, Trace vitamins and NaHCO3, adjust pH to 6.8 and autoclave. Steam sulfur for 3 hr on each of 3 successive days. Filter-sterilize Trace vitamins and 5% NaHCO3 solution, and add to the medium. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) containing sulfur under a stream of N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Replace the gas phase with N2-CO2-O2 (79:20:1, v/v) and pressurized inoculated bottles to 200 kPa with the same gas mixture.

Trace mineral solution #

Solution occurs in 1 other media (first defined in Medium J508).
1 Use Trace mineral solution of Medium No. 413 with 0.01 g/L (final) Na2MoO4 x 2H2O.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.782 338.508
MgSO4 x 7 H2O 3.956 16.052
Sulfur 2.967 92.528
MgCl2 x 6 H2O 2.967 14.596
NaHCO3 1.978 23.549
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Cultivation metadata from 2 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 2 strains
oxygen microaerophile 1 strain
temperature thermophilic 2 strains