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JCM 512 . A1 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J512 #

ml
Equipment needed: Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 19.608 335.521
KCl 0.77 g 0.755 10.126
NH4Cl 0.25 g 0.245 4.582
KH2PO4 0.10 g 0.098 0.72
MgSO4 x 7 H2O 0.20 g 0.196 0.796
DL vitamins (refrigerated) 10.00 ml - -
DL minerals (refrigerated) 10.00 ml - -
NaHCO3 2.00 g 1.961 23.341
Distilled water 1000.00 ml - -
1 Mix ingredients except NaHCO3, adjust pH to 6.8 and add NaHCO3 to the medium. Dispense the medium (9 ml) into 26-ml anaerobic pressure tubes under a stream of oxygen-free N2-CO2 (80:20) and seal. After autoclaving, aseptically and anaerobically add 0.2 ml APM salts solution and 0.1 ml Sulfide stock solution and 0.1 ml Galactose stock solution.

DL minerals (refrigerated) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid , trisodium salt 1.500 g 1.5 7.848
MgSO4 x 7 H2O 3.000 g 3 12.172
MnSO4 x n H2O 0.500 g 0.5 -
NaCl 1.000 g 1 17.112
FeSO4 x 7 H2O 0.100 g 0.1 0.36
CaCl2 x 2 H2O 0.100 g 0.1 0.68
CoCl2 x 6 H2O 0.100 g 0.1 0.77
ZnCl2 0.130 g 0.13 0.954
CuSO4 x 5 H2O 0.010 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.010 g 0.01 0.021
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.025 g 0.025 0.103
NiCl2 x 6 H2O 0.024 g 0.024 0.185
Na2WO4 x 2 H2O 0.025 g 0.025 0.076
Distilled water 1000.000 ml - -

DL vitamins (refrigerated) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.00 mg 0.002 0.008
Pantothenic acid 5.00 mg 0.005 0.023
Vitamin B12 0.10 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.00 mg 0.005 0.036
Thioctic acid 5.00 mg 0.005 0.024
Nicotinic acid 5.00 mg 0.005 0.041
Thiamine HCl 5.00 mg 0.005 -
Riboflavin 5.00 mg 0.005 0.013
Pyridoxine hydrochloride 0.01 g 0.01 -
Folic acid 2.00 mg 0.002 0.005
Distilled water 1000.00 ml - -

APM salts solution (autoclaved) #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 21.20 g 212 1042.78
CaCl2 x 2 H2O 3.04 g 30.4 206.782
Distilled water 100.00 ml - -

Sulfide stock solution (autoclaved) #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.4 g 40 166.54
Distilled water 10.0 ml - -

Galactose stock solution (filter-sterilized) #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Galactose 0.901 g 90.1 500.111
Distilled water 10.000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8
Equipment needed: Anaerobic workbench
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.618 335.689
NaHCO3 1.961 23.341
KCl 0.755 10.126
NH4Cl 0.245 4.582
MgSO4 x 7 H2O 0.225 0.915
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen facultative anaerobe 1 strain
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