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J513. CALDOCOCCUS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.10 g 0.099 1.692
2 0.10 g 0.099 0.673
3 0.05 g 0.049 0.178
4
(BD-Difco)
0.10 g 0.099 -
5
(BD-Difco)
0.10 g 0.099 -
6 0.80 g 0.791 -
7 Trace vitamins 10.00 ml - -
8 Trace mineral solution 1.00 ml - -
9 2.00 g 1.978 61.693
10 1.00 mg 9.9e-4 0.004
11 0.50 g 0.495 2.059
12 1000.00 ml 987.346 54806
Instructions:
  1. Mix ingredients except vitamins, sulfur and Na2S x 9H2O, adjust pH to 2.7 with H2SO4 and autoclave. Separately autoclave 5%(w/v) Na2S x 9H2O solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Filter-sterilize Trace vitamins solution. Distribute the medium into culture vessels (e.g., 20 ml per 100 ml serum bottles) containing Trace vitamins and sulfur, replace the gas phase with a H2-CO2 (80:20) gas atmosphere and seal with butyl rubber stoppers. Prior to inoculation, add sterile Na2S x 9H2O solution and adjust pH to 3.0 with sterile 1 N H2SO4. Pressurize the inoculated bottle to 200 kPa H2-CO2 (80:20).
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.0 g 5 -
2 1.0 g 1 -
3 1.0 g 1 -
4 0.1 g 0.1 -
5 0.1 g 0.1 1.617
6 0.1 g 0.1 0.413
7 1000.0 ml 998.207 55408.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.9
Sulfur 1.978 61.693
Peptone 0.791 -
Na2S x 9H2O 0.495 2.059
NaCl 0.099 1.692
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