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JCM 513 . CALDOCOCCUS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J513 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 0.10 g 0.099 1.693
CaCl2 x 2 H2O 0.10 g 0.099 0.673
FeSO4 x 7 H2O 0.05 g 0.049 0.178
Yeast extract
(BD-Difco) 		0.10 g 0.099 -
Casamino acids
(BD-Difco) 		0.10 g 0.099 -
Peptone 0.80 g 0.791 -
Trace vitamins 10.00 ml - -
Trace mineral solution 1.00 ml - -
Sulfur 2.00 g 1.978 61.685
Resazurin 1.00 mg 9.9e-4 0.004
Na2S x 9 H2O 0.50 g 0.495 2.059
Distilled water 1000.00 ml - -
1 Mix ingredients except vitamins, sulfur and Na2S x 9H2O, adjust pH to 2.7 with H2SO4 and autoclave. Separately autoclave 5% (w/v) Na2S x 9H2O solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Filter-sterilize Trace vitamins solution. Distribute the medium into culture vessels (e.g., 20 ml per 100 ml serum bottles) containing Trace vitamins and sulfur, replace the gas phase with a H2-CO2 (80:20) gas atmosphere and seal with butyl rubber stoppers. Prior to inoculation, add sterile Na2S x 9H2O solution and adjust pH to 3.0 with sterile 1 N H2SO4. Pressurize the inoculated bottle to 200 kPa H2-CO2 (80:20).

Trace mineral solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnSO4 x n H2O 5.0 g 5 -
CoSO4 x 7 H2O 1.0 g 1 -
ZnSO4 x 7 H2O 1.0 g 1 3.478
CuSO4 x 5 H2O 0.1 g 0.1 0.401
H3BO3 0.1 g 0.1 1.617
Na2MoO4 x 2 H2O 0.1 g 0.1 0.413
Distilled water 1000.0 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 2.7
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulfur 1.978 61.685
Peptone 0.791 -
Na2S x 9 H2O 0.495 2.059
NaCl 0.099 1.693
CaCl2 x 2 H2O 0.099 0.673
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature hyperthermophilic 1 strain