Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Glucose | 0.15 | g | 0.15 | 0.833 | |
Yeast extract | 1.00 | g | 1 | - | |
(NH4)2SO4 | 0.50 | g | 0.5 | 3.784 | |
CaCO3 | 0.10 | g | 0.1 | 0.999 | |
Ca(NO3)2 | 0.10 | g | 0.1 | 0.609 | |
KCl | 0.05 | g | 0.05 | 0.671 | |
K2HPO4 | 0.05 | g | 0.05 | 0.287 | |
MgSO4 x 7 H2O | 0.05 | g | 0.05 | 0.203 | |
Na2S x 9 H2O | 0.20 | g | 0.2 | 0.833 | |
Vitamin solution | 10.00 | ml | - | - | |
Agar | 15.00 | g | 15 | 44.599 | |
Distilled water | 990.00 | ml | - | - | |
1 Add components except Vitamin solution to distilled water and bring volume to 990 ml. Adjust pH to 7.3. After autoclaving, aseptically add filter-sterilized Vitamin solution. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.00 | mg | 0.002 | 0.008 |
Folic acid | 2.00 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.00 | mg | 0.01 | - |
Thiamine-HCl x 2 H2O | 5.00 | mg | 0.005 | - |
Riboflavin | 5.00 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.00 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.00 | mg | 0.005 | 0.01 |
p-Aminobenzoic acid | 1.00 | mg | 1.0e-3 | 0.007 |
Myo-inositol | 1.00 | mg | 1.0e-3 | 0.006 |
Vitamin B12 | 0.01 | mg | 1.0e-5 | 7.4e-6 |
Distilled water | 1000.00 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.3 |
Equipment needed: | Filter |
Edit in Medium builder |
All strains for this medium: | 3 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Agar | 15 | 44.599 |
Yeast extract | 1 | - |
(NH4)2SO4 | 0.5 | 3.784 |
Na2S x 9 H2O | 0.2 | 0.833 |
Glucose | 0.15 | 0.833 |
Show more |