Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Mineral solution | 50.0 | ml | - | - | |
FeCl2 solution | 1.0 | ml | - | - | |
Trace element solution | 1.0 | ml | - | - | |
Rumen fluid
, clarified
(see Medium No. 266) |
50.0 | ml | - | - | |
Trypticase peptone
(BD-BBL) |
1.0 | g | 1.109 | - | |
Vitamin solution | 5.0 | ml | - | - | |
Butyric acid | 1.7 | g | 1.885 | 21.39 | |
Na2SO4 | 2.8 | g | 3.104 | 21.855 | |
Resazurin | 1.0 | mg | 0.001 | 0.005 | |
Distilled water | 795.0 | ml | - | - | |
1 Mix components, adjust pH to 7.2, bring to a boil for a few seconds and cool down under a stream of a N2-CO2 (80:20) gas mixture. Distribute Solution A into culture vessels (e.g., 9 ml per tube) under the same gas mixture, seal with butyl rubber stoppers and autoclave. | |||||
2 To complete the medium, add 0.1 volume of 3.5% NaHCO3 solution [filter-sterilized and stored under a N2-CO2 (80:20) gas mixture] and reduce the medium with 0.3 g/L (final) L-cysteine x HCl x H2O and 0.3 g/L (final) Na2S x 9H2O (autoclave and store 3% stock solutions under a N2 atmosphere). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
KH2PO4 | 10.0 | g | 10 | 73.483 |
MgCl2 x 6 H2O | 6.6 | g | 6.6 | 32.464 |
NaCl | 8.0 | g | 8 | 136.893 |
NH4Cl | 8.0 | g | 8 | 149.561 |
CaCl2 x 2 H2O | 1.0 | g | 1 | - |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 0.25 | mg | 2.5e-4 | 0.001 |
Nicotinic acid | 2.50 | mg | 0.003 | 0.02 |
Thiamine HCl | 1.25 | mg | 0.001 | - |
p-Aminobenzoic acid | 1.25 | mg | 0.001 | 0.009 |
DL-Calcium pantothenate | 0.62 | mg | 6.2e-4 | 0.001 |
Pyridoxine hydrochloride | 6.20 | mg | 0.006 | - |
Distilled water | 1000.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Last modified: | 03.04.22 |
Source: | JCM |
Medium type: | Complex medium |
Final pH: | 7.2 |
Equipment needed: | Autoclave, Filter |
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