Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 1.000 | g | 0.993 | 16.993 | |
KH2PO4 | 0.014 | g | 0.014 | 0.102 | |
CaCl2 x 2 H2O | 0.080 | g | 0.079 | 0.54 | |
MgSO4 x 7 H2O | 0.340 | g | 0.338 | 1.37 | |
MgCl2 x 6 H2O | 0.420 | g | 0.417 | 2.052 | |
KCl | 0.033 | g | 0.033 | 0.44 | |
NH4Cl | 0.500 | g | 0.497 | 9.283 | |
Na2SiO3 x 9 H2O | 0.500 | g | 0.497 | - | |
Fe2(SO4)3 x n H2O | 20.000 | mg | 0.02 | - | |
Na2SeO3 x 5 H2O | 0.500 | mg | 5.0e-4 | 0.002 | |
NiCl2 x 6 H2O | 0.500 | mg | 5.0e-4 | 0.004 | |
Trace mineral solution | 3.000 | ml | - | - | |
Distilled water | 1000.000 | ml | - | - | |
1 Mix components thoroughly and autoclave. After cooling, add the following solutions (filter-sterilized) to the medium: | |||||
Trace vitamins | 1.000 | ml | - | - | |
NaHCO3
(8%) |
2.000 | ml | - | - | |
CuSO4
(1 mM) |
1.000 | ml | - | - | |
2 Then, distribute the medium into culture vessels (leave >80% gas phase, by volume) and seal with butyl rubber stoppers. Replace the gas phase with N2-CO2-CH4-O2 (40:15:40:5, by volume) and pressurize to 100 kPa with the same gas mixture. Check final pH of the medium to be around 6.0. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
CoSO4 x 7 H2O | 0.18 | g | 0.18 | 1.161 | |
ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 | 0.02 | g | 0.02 | 0.077 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 6.5 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 0.993 | 16.993 |
NH4Cl | 0.497 | 9.283 |
Na2SiO3 x 9 H2O | 0.497 | - |
MgCl2 x 6 H2O | 0.417 | 2.052 |
MgSO4 x 7 H2O | 0.338 | 1.37 |
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