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JCM 523 . METHYLOTHERMUS SUBTERRANEUS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J523 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.000 g 0.993 16.993
KH2PO4 0.014 g 0.014 0.102
CaCl2 x 2 H2O 0.080 g 0.079 0.54
MgSO4 x 7 H2O 0.340 g 0.338 1.37
MgCl2 x 6 H2O 0.420 g 0.417 2.052
KCl 0.033 g 0.033 0.44
NH4Cl 0.500 g 0.497 9.283
Na2SiO3 x 9 H2O 0.500 g 0.497 -
Fe2(SO4)3 x n H2O 20.000 mg 0.02 -
Na2SeO3 x 5 H2O 0.500 mg 5.0e-4 0.002
NiCl2 x 6 H2O 0.500 mg 5.0e-4 0.004
Trace mineral solution 3.000 ml - -
Distilled water 1000.000 ml - -
1 Mix components thoroughly and autoclave. After cooling, add the following solutions (filter-sterilized) to the medium:
Trace vitamins 1.000 ml - -
NaHCO3
(8%) 		2.000 ml - -
CuSO4
(1 mM) 		1.000 ml - -
2 Then, distribute the medium into culture vessels (leave >80% gas phase, by volume) and seal with butyl rubber stoppers. Replace the gas phase with N2-CO2-CH4-O2 (40:15:40:5, by volume) and pressurize to 100 kPa with the same gas mixture. Check final pH of the medium to be around 6.0.

Trace mineral solution #

1000
Solution occurs in 1 other media (first defined in Medium J523).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MnSO4 x n H2O 0.50 g 0.5 -
CoSO4 x 7 H2O 0.18 g 0.18 1.161
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.02 g 0.02 0.077
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 0.993 16.993
NH4Cl 0.497 9.283
Na2SiO3 x 9 H2O 0.497 -
MgCl2 x 6 H2O 0.417 2.052
MgSO4 x 7 H2O 0.338 1.37
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain