Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 30.00 | g | 29.528 | 505.263 | |
KH2PO4 | 0.14 | g | 0.138 | 1.013 | |
CaCl2 x 2 H2O | 0.80 | g | 0.787 | 5.356 | |
MgSO4 x 7 H2O | 3.40 | g | 3.346 | 13.577 | |
MgCl2 x 6 H2O | 4.20 | g | 4.134 | 20.334 | |
KCl | 0.33 | g | 0.325 | 4.357 | |
NaNO3 | 0.25 | g | 0.246 | 2.895 | |
NH4Cl | 0.25 | g | 0.246 | 4.6 | |
Na2SiO3 x 9 H2O | 0.20 | g | 0.197 | - | |
Fe2(SO4)3 x n H2O | 20.00 | mg | 0.02 | - | |
Na2SeO3 x 5 H2O | 0.50 | mg | 4.9e-4 | 0.002 | |
NiCl2 x 6 H2O | 0.50 | mg | 4.9e-4 | 0.004 | |
Trace mineral solution | 3.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix components, adjust pH to 6.0 - 6.5 and autoclave. After cooling, add the following solutions (filter-sterilized) to the medium: | |||||
Trace vitamins | 1.00 | ml | - | - | |
NaHCO3
(5%) |
10.00 | ml | - | - | |
CuSO4
(1 mM) |
2.00 | ml | - | - | |
2 Distribute the medium into culture vessels (leave >80% volume gas phase) under a N2-CO2 (4:1) gas mixture and seal with butyl rubber stoppers. Pressurize the culture vessels to 200 kPa with methane gas. Finally add O2 gas to make up 2% O2 concentration in the gas phase. Readjust pH to 6.0 - 6.3, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
CoSO4 x 7 H2O | 0.18 | g | 0.18 | 1.161 | |
ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 | 0.02 | g | 0.02 | 0.077 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 6.2 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 2 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 29.528 | 505.263 |
MgCl2 x 6 H2O | 4.134 | 20.334 |
MgSO4 x 7 H2O | 3.346 | 13.577 |
CaCl2 x 2 H2O | 0.787 | 5.356 |
KCl | 0.325 | 4.357 |
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