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J536. PHE91 MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(see below)
969 ml - -
2 SL-7 trace element solution 1 ml - -
3 Vitamin solution 1 ml - -
4 Vitamin B12 solution 1 ml - -
5 Selenite-tungstate solution 1 ml - -
6
(68 mM)
1 ml - -
7
(609 mM)
10 ml - -
8
(0.02%)
1 ml - -
9
(0.2 M)
5 ml - -
10
(1 M)
10 ml - -
Instructions:
  1. Autoclave the basal solution under a N2 gas atmosphere. Separately filter-sterilize the other solutions and add to the medium anaerobically and aseptically. Prepare dithionite solution just before use.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.46 g 0.475 4.127
2 0.49 g 0.506 2.052
3 969.00 ml 998.207 55408.8
Solution occurs in 1 other media (first defined in medium J536 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(7.7 M, 25%)
10.0 ml 3.139 86.082
2 1.5 g 1.485 -
3 190.0 mg 0.188 -
4 100.0 mg 0.099 -
5 70.0 mg 0.069 0.509
6 62.0 mg 0.061 0.993
7 36.0 mg 0.036 0.147
8 24.0 mg 0.024 -
9 17.0 mg 0.017 -
10 1000.0 ml 988.324 54860.2
Instructions:
  1. Initially, dissolve FeCl2 x 4H2O in 25% HCl, then add distilled water followed by the other components.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.5 mg 0.003 0.01
2 25.0 mg 0.025 0.203
3 12.5 mg 0.013 -
4 12.5 mg 0.013 0.091
5 6.5 mg 0.007 -
6 62.5 mg 0.063 -
7 1000.0 ml 998.207 55408.8
Instructions:
  1. Store in the dark at 4C.
Solution occurs in 69 other media (first defined in medium J431 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 0.4 10.001
2 6.0 mg 0.006 -
3 8.0 mg 0.008 -
4 1000.0 ml 998.207 55408.8
Solution occurs in 18 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 mg 0.05 0.032
2 100 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 3.983 221.087
HCl 0.003 0.086
FeCl2 x 4H2O 0.001 -
NaOH 4.0e-4 0.01
CoCl2 x 6H2O 1.9e-4 -
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