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JCM 536 . PHE91 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J536 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Basal solution 969 ml - -
SL-7 trace element solution 1 ml - -
Vitamin solution 1 ml - -
Vitamin B12 solution 0.05 g/L 1 ml - -
Selenite-tungstate solution 1 ml - -
CaCl2 x 2 H2O
(68 mM) 		1 ml - -
K2HPO4
(609 mM) 		10 ml - -
Resazurin
(0.02%) 		1 ml - -
Dithionite
(0.2 M) 		5 ml - -
Pyruvate
(1 M) 		10 ml - -
1 Autoclave the basal solution under a N2 gas atmosphere. Separately filter-sterilize the other solutions and add to the medium anaerobically and aseptically. Prepare dithionite solution just before use.

Basal solution #

969
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4H2PO4 0.46 g 0.475 4.127
MgSO4 x 7 H2O 0.49 g 0.506 2.052
Distilled water 969.00 ml - -

SL-7 trace element solution #

1010
Solution occurs in 1 other media (first defined in Medium J536).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml - -
FeCl2 x 4 H2O 1.5 g 1.485 7.47
CoCl2 x 6 H2O 190.0 mg 0.188 1.449
MnCl2 x 4 H2O 100.0 mg 0.099 -
ZnCl2 70.0 mg 0.069 0.508
H3BO3 62.0 mg 0.061 0.993
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.147
NiCl2 x 6 H2O 24.0 mg 0.024 0.183
CuCl2 x 2 H2O 17.0 mg 0.017 0.099
Distilled water 1000.0 ml - -
1 Initially, dissolve FeCl2 x 4H2O in 25% HCl, then add distilled water followed by the other components.

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.5 mg 0.003 0.01
Nicotinic acid 25.0 mg 0.025 0.203
Thiamine HCl 12.5 mg 0.013 -
p-Aminobenzoic acid 12.5 mg 0.013 0.091
DL-Calcium pantothenate 6.5 mg 0.007 0.014
Pyridoxine hydrochloride 62.5 mg 0.063 -
Distilled water 1000.0 ml - -
1 Store in the dark at 4C.

Vitamin B12 solution 0.05 g/L #

100
Solution occurs in 18 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 5 mg 0.05 0.037
Distilled water 100 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
FeCl2 x 4 H2O 0.001 0.007
NaOH 4.0e-4 0.01
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 9.9e-5 -
ZnCl2 6.9e-5 5.1e-4
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
oxygen facultative anaerobe 1 strain
temperature mesophilic 1 strain