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JCM 537 . TREPONEMA ISOPTEROCOLA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J537 #

ml
Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.00 g 0.999 17.095
KCl 0.50 g 0.5 6.7
MgCl2 x 6 H2O 0.40 g 0.4 1.966
CaCl2 x 2 H2O 0.10 g 0.1 0.68
NH4Cl 0.30 g 0.3 5.603
KH2PO4 0.20 g 0.2 1.468
Na2SO4 0.15 g 0.15 1.055
Yeast extract 4.00 g 3.996 -
Casein peptone 2.00 g 1.998 -
Resazurin 0.50 mg 5.0e-4 0.002
Distilled water 1000.00 ml - -
1 Prepare the medium anaerobically, dispense its 5 ml each in anaerobic tubes under a N2-CO2 atmosphere (80:20) and autoclave. Add the following solutions via injection from sterile anaerobic stock solutions per 5 ml:
Trace element solution SL-10 , 10-fold diluted
(*) 		0.05 ml - -
Vitamin solution , 10-fold diluted
(**) 		0.05 ml - -
Co-factors solution
(***) 		0.10 ml - -
NaHCO3
(10%, w/v) 		0.30 ml - -
Sugar
(250 mM each of xylose, maltose and cellobiose) 		0.10 ml - -
Dithiothreitol
(0.25 M) 		0.05 ml - -
2 Adjust pH to 7.2 - 7.4.

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 -
Thiamine-HCl x 2 H2O 200 mg 0.2 -
Distilled water 1000 ml - -

Co-factors solution #

1000
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Pyridoxal hydrochloride 250.0 mg 0.25 -
Pyridoxal phosphate 250.0 mg 0.25 1.012
Ca-folinate 50.0 mg 0.05 -
beta-NAD 50.0 mg 0.05 0.075
Coenzyme A 50.0 mg 0.05 0.065
FAD 50.0 mg 0.05 0.064
Nicotinamide 25.0 mg 0.025 0.205
Folic acid 2.5 mg 0.003 0.006
Riboflavin 0.5 mg 5.0e-4 0.001
Hemin
(dissolved separately in 10 mM NaOH) 		65.0 mg 0.065 0.1
Thiamine pyrophosphate 2.5 g 2.5 5.426
Distilled water 1000.0 ml - -
1 Adjust pH to 7.0 with 1 N NaOH and filter-sterilize.

Trace element solution SL-10 #

1000
Solution occurs in 20 other media (first defined in Medium J433).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%, 7.7 M) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 3.996 -
Casein peptone 1.998 -
NaCl 0.999 17.095
KCl 0.5 6.7
MgCl2 x 6 H2O 0.4 1.966
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain