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JCM 540 . MODIFIED SYNTROPHOMONAS MEDIUM FOR OL4

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Solution A #

802
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Mineral solution 50.0 ml - -
FeCl2 solution 1.0 ml - -
Trace element solution 1.0 ml - -
Vitamin solution 5.0 ml - -
Na2SO4 2.8 g 3.491 24.58
Resazurin 1.0 mg 0.001 0.005
Distilled water 745.0 ml - -
1 Mix components, adjust pH to 7.2, bring to a boil for a few seconds and cool down under a stream of a N2-CO2 (80:20) gas mixture. Distribute Solution A into culture vessels (e.g., 8 ml per tube) under the same gas mixture, seal with butyl rubber stoppers and autoclave.

Solution B #

100
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sodium oleate 0.15 g 1.5 4.927
Distilled water 100.00 ml - -
1 Autoclave and store under a N2 atmosphere.
2 To complete the medium, combine 0.8 volume of Solution A, 0.1 volume of Solution B and 0.1 volume of 3.5% NaHCO3 solution [filter-sterilized and stored under a N2-CO2 (80:20) gas mixture]. Reduce the medium with 0.3 g/L (final) L-cysteine x HCl x H2O and 0.3 g/L (final) Na2S x 9H2O (autoclave and store 3% stock solutions under a N2 atmosphere).
3 Comment: For strain JCM 13948, incubate with a 10% inoculum together with a 5% inoculum of Methanobacterium formicicum NT (=JCM 10132).

Mineral solution #

1000
Solution occurs in 2 other media (first defined in Medium J519).
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 10.0 g 10 73.483
MgCl2 x 6 H2O 6.6 g 6.6 32.464
NaCl 8.0 g 8 136.893
NH4Cl 8.0 g 8 149.561
CaCl2 x 2 H2O 1.0 g 1 -
Distilled water 1000.0 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Vitamin solution #

1000
Solution occurs in 2 other media (first defined in Medium J519).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 0.25 mg 2.5e-4 0.001
Nicotinic acid 2.50 mg 0.003 0.02
Thiamine HCl 1.25 mg 0.001 -
p-Aminobenzoic acid 1.25 mg 0.001 0.009
DL-Calcium pantothenate 0.62 mg 6.2e-4 0.001
Pyridoxine hydrochloride 6.20 mg 0.006 -
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: No strains found