Name: TINDALLIA TEXCOCONENSIS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Main sol. J543 #

Solution A #

710

Equipment needed: Autoclave Anaerobic workbench

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NH₄Cl	1.0	g	1.408	26.331
KH₂PO₄	0.3	g	0.423	3.105
KCl	0.1	g	0.141	1.889
CaCl₂	0.1	g	0.141	1.269
MgCl₂ x 6 H₂O	0.1	g	0.141	0.693
NaCl	75.0	g	105.634	1807.56
Yeast extract	0.5	g	0.704	-
Peptone	5.0	g	7.042	-
Resazurin	1.0	mg	0.001	0.006
Trace minerals	10.0	ml	-	-
Distilled water	700.0	ml	-	-
1 Mix components and adjust pH to 9.0 with 10 N KOH. Autoclave the solution anaerobically under a N₂ atmosphere.

300

Equipment needed: Anaerobic workbench

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaHCO₃	20	g	66.667	793.585
Na₂CO₃	3	g	10	94.35
Distilled water	300	ml	-	-
1 Prepare the medium anaerobically under a N₂ atmosphere.
2 To complete the medium, combine Solutions A and B under a N₂ atmosphere. Prior to inoculation, add 0.5 g/L (final) L-cysteine x HCl x H₂O and 0.4 g/L (final) Na₂S x 9H₂O from anaerobic and sterile stock solutions (5.0%).

1000

Solution occurs in 97 other media (first defined in Medium J151).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MgSO₄ x 7 H₂O	3.00	g	3	12.172
MnSO₄ x n H₂O	0.50	g	0.5	-
NaCl	1.00	g	1	17.112
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
CoSO₄ x 7 H₂O	0.10	g	0.1	0.645
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂	0.01	g	0.01	0.039
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
Distilled water	1000.00	ml	-	-
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	9.0
Equipment needed:	Anaerobic workbench, Autoclave

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