Name: THERMODESULFOVIBRIO HYDROGENIPHILUS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J544 #

Equipment needed: Autoclave Hungate tubes Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.30	g	0.3	2.202
K₂HPO₄	0.30	g	0.3	1.721
NH₄Cl	1.00	g	0.999	18.676
NaCl	1.00	g	0.999	17.095
Na₂SO₄	3.00	g	2.997	21.1
KCl	0.10	g	0.1	1.34
CaCl₂	0.10	g	0.1	0.9
MgCl₂ x 6 H₂O	0.50	g	0.5	2.457
Yeast extract	0.10	g	0.1	-
Sodium acetate	0.16	g	0.16	1.949
Trace element solution	1.00	ml	-	-
NaHCO₃	2.00	g	1.998	23.784
L-Cysteine HCl x H₂O	0.50	g	0.5	-
Na₂S x 9 H₂O	0.40	g	0.4	1.664
Resazurin	1.00	mg	1.0e-3	0.004
Distilled water	1000.00	ml	-	-
1 Mix ingredients except NaHCO₃, L-cysteine x HCl x H₂O and Na₂S x 9H₂O and adjust pH to 7.2. Distribute the medium into tubes (e.g., 5 ml of the medium in the Hungate tubes) under a H₂-CO₂ gas mixture (4:1, v/v), seal with butyl rubber stoppers and autoclave. After cooling, add solutions of NaHCO₃ [5%, filter-sterilized and stored under a N₂-CO₂ (4:1, v/v) gas mixture], L-cysteine x HCl x H₂O and Na₂S x 9H₂O (5%, autoclaved and stored under N₂) to the medium. Pressurize the inoculated tubes to 200 kPa H₂-CO₂ (4:1, v/v).

Trace element solution #

1000

Solution occurs in 38 other media (first defined in Medium J439).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%)	12.5	ml	-	-
FeSO₄ x 7 H₂O	2.1	g	2.1	7.554
H₃BO₃	30.0	mg	0.03	0.485
MnCl₂ x 4 H₂O	100.0	mg	0.1	-
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
ZnSO₄ x 7 H₂O	144.0	mg	0.144	0.501
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	987.0	ml	-	-

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	7.2
Equipment needed:	Autoclave, Filter, Hungate tubes

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