Name: AMINIPHILUS CIRCUMSRIPTUS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J550 #

Equipment needed: Hungate tubes Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.3	g	0.297	2.183
K₂HPO₄	0.3	g	0.297	1.705
NH₄Cl	1.0	g	0.99	18.51
NaCl	10.0	g	9.901	169.421
KCl	0.1	g	0.099	1.328
CaCl₂	0.1	g	0.099	0.892
MgCl₂ x 6 H₂O	0.5	g	0.495	2.435
Trace minerals	10.0	ml	-	-
Yeast extract	2.0	g	1.98	-
Peptone	10.0	g	9.901	-
L-Cysteine HCl x H₂O	0.5	g	0.495	-
Distilled water	1000.0	ml	-	-
1 Mix ingredients and adjust pH to 7.0. Distribute into tubes (e.g., 5 ml of the medium in the Hungate tubes) under a N₂ atmosphere, seal with butyl rubber stoppers and autoclave.

Trace minerals #

1000

Solution occurs in 97 other media (first defined in Medium J151).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MgSO₄ x 7 H₂O	3.00	g	3	12.172
MnSO₄ x n H₂O	0.50	g	0.5	-
NaCl	1.00	g	1	17.112
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
CoSO₄ x 7 H₂O	0.10	g	0.1	0.645
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂	0.01	g	0.01	0.039
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
Distilled water	1000.00	ml	-	-
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

All strains for this medium:

CSV

JSON