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JCM 552 . SULFATE REDUCING BACTERIUM MEDIUM WITH LACTATE

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J552 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.5 g 0.499 3.663
NH4Cl 1.0 g 0.997 18.639
Na2SO4 1.0 g 0.997 7.019
CaCl2 x 2 H2O 0.1 g 0.1 0.678
MgSO4 x 7 H2O 2.0 g 1.994 8.09
NaCl 10.0 g 9.97 170.604
Yeast extract
(BD-Difco) 		0.5 g 0.499 -
Vitamin solution 1.0 ml - -
FeCl2 solution 1.0 ml - -
Trace element solution 1.0 ml - -
L-Sodium lactate 2.3 g 2.293 20.463
Resazurin 1.0 mg 1.0e-3 0.004
L-Cysteine HCl x H2O 0.5 g 0.499 -
Distilled water 1000.0 ml - -
1 Mix ingredients except L-sodium lactate and L-cysteine x H2O x HCl, adjust pH to 7.5 and autoclave under a N2 atmosphere. Separately autoclave L-sodium lactate and L-cysteine x H2O x HCl in 10 ml distilled water under a N2 atmosphere and add to the medium. Readjust pH to 7.5 with 1 N NaOH (from a sterile stock solution).
2 For preparation of solid medium, add 15 g/L agar (BD-Difco).

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 10 �g - -
p-Aminobenzoic acid 40 �g - -
Nicotinic acid 100 �g - -
Distilled water 1000 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 9.97 170.604
L-Sodium lactate 2.293 20.463
MgSO4 x 7 H2O 1.994 8.09
NH4Cl 0.997 18.639
Na2SO4 0.997 7.019
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain