J563
563 .
TREPONEMA ZIOLECKII MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J563 #
ml
Equipment needed: Autoclave
Hungate tubes
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trypticase peptone | 1.0 | g | 1 | - | |
| Yeast extract | 2.5 | g | 2.5 | - | |
|
Starch
(soluble) |
2.0 | g | 2 | - | |
| Cellobiose | 2.0 | g | 2 | 5.843 | |
| NaHCO3 | 4.0 | g | 4 | 47.615 | |
| L-Cysteine HCl x H2O | 1.0 | g | 1 | - | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
|
Vitamin K1
(1 mg/ml) |
0.2 | ml | - | - | |
| Mineral solution 1 | 150.0 | ml | - | - | |
| Mineral solution | 150.0 | ml | - | - | |
| Clarified rumen fluid | 300.0 | ml | - | - | |
| Distilled water | 400.0 | ml | - | - | |
| 1 Dissolve ingredients except NaHCO3 in distilled water, boil for 5 min, then cool to room temperature while gassing with 100% CO2. Add NaHCO3, equilibrate the medium with NaOH to pH 6.9. Distribute 10 ml amounts in anaerobic Hungate tubes under CO2 and autoclave. | |||||
Solution occurs in 4
other media (first defined in Medium 203).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| K2HPO4 | 6 | g | 6 | 34.448 |
| Distilled water | 1000 | ml | - | - |
Solution occurs in 4
other media (first defined in Medium 330).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| KH2PO4 | 6.0 | g | 6 | 44.09 |
| NaCl | 12.0 | g | 12 | 205.339 |
| (NH4)2SO4 | 6.0 | g | 6 | 45.406 |
| CaCl2 x 2 H2O | 1.6 | g | 1.6 | 10.883 |
| MgSO4 x 7 H2O | 2.5 | g | 2.5 | 10.143 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Anaerobic workbench, Autoclave, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .