Name: DESULFOVIBRIO HEB233 MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Main sol. J565 #

Solution A #

902

Equipment needed: Autoclave Anaerobic workbench Hungate tubes Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.3	g	0.333	2.444
K₂HPO₄	0.3	g	0.333	1.91
NH₄Cl	1.0	g	1.109	20.726
Na₂SO₄	3.0	g	3.326	23.416
NaCl	23.0	g	25.499	436.326
KCl	0.1	g	0.111	1.487
CaCl₂	0.1	g	0.111	0.999
Yeast extract (BD-Difco)	0.1	g	0.111	-
Trace element solution	1.0	ml	-	-
L-Cysteine HCl x H₂O	0.5	g	0.554	-
Resazurin	1.0	mg	0.001	0.005
Distilled water	900.0	ml	-	-
1 To complete the medium, add the following stock solutions [Filter-sterilize NaHCO₃ solution and store under a N₂-CO₂ (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N₂ atmosphere] to the medium (per 4.5 ml):
Sodium lactate (1 M)	0.1	ml	-	-
MgCl₂ x 6 H₂O (15%)	0.1	ml	-	-
NaHCO₃ (5%)	0.2	ml	-	-
Na₂S x 9 H₂O (2%)	0.1	ml	-	-
2 Mix ingredients and adjust pH to 7.2. Distribute 0.9 volume of the medium into tubes (e.g., 4.5 ml of the medium in the Hungate tubes) under a N₂-CO₂ (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave at 110C for 45 min.

1000

Solution occurs in 38 other media (first defined in Medium J439).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%)	12.5	ml	-	-
FeSO₄ x 7 H₂O	2.1	g	2.1	7.554
H₃BO₃	30.0	mg	0.03	0.485
MnCl₂ x 4 H₂O	100.0	mg	0.1	-
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
ZnSO₄ x 7 H₂O	144.0	mg	0.144	0.501
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	987.0	ml	-	-

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	7.2
Equipment needed:	Anaerobic workbench, Autoclave, Filter, Hungate tubes

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