J566
566 .
ILYOBACTER MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J566 #
ml
Equipment needed: Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 20.00 | g | 9.901 | 169.421 | |
| KCl | 0.77 | g | 0.381 | 5.113 | |
| CaCl2 x 2 H2O | 0.02 | g | 0.01 | 0.067 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.248 | 1.004 | |
| NH4Cl | 1.00 | g | 0.495 | 9.255 | |
| Wolfe's mineral solution | 10.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
| KNO3 | 1.00 | g | 0.495 | 4.896 | |
| KH2PO4 x 2 H2O | 0.50 | g | 0.248 | - | |
| Yeast extract | 0.50 | g | 0.248 | - | |
| Peptone | 0.50 | g | 0.248 | - | |
| Sodium acetate | 0.10 | g | 0.05 | 0.603 | |
| Sodium succinate | 0.10 | g | 0.05 | 0.305 | |
| DL-Sodium malate | 0.10 | g | 0.05 | 0.278 | |
| Sodium pyruvate | 0.10 | g | 0.05 | 0.45 | |
| Glucose | 1.00 | g | 0.495 | 2.748 | |
| L-Cysteine HCl x H2O | 0.30 | g | 0.149 | - | |
|
Resazurin
(1%) |
100.00 | �l | - | - | |
| Distilled water | 2000.00 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
| 2 Boil until the volume decreases to 1.0 L and dispense under an atmosphere of N2 into 15 ml Hungate tube. If the medium stays pink (O2 is present), add one drop of 5% Na2S from the sterile stock solution. | |||||
Solution occurs in 7
other media (first defined in Medium J265).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Trace minerals | 1000.00 | ml | - | - |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 |
| Na2SeO3 | 1.00 | mg | 1.0e-3 | - |
| Na2WO4 x 2 H2O | 0.01 | g | 0.01 | 0.03 |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Hungate tubes |
| Edit in Medium builder | |