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JCM 567 . VENENIVIBRIO STAGNISPUMANTIS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J567 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.50 g 0.495 6.634
MgCl2 x 6 H2O 1.36 g 1.345 6.617
MgSO4 x 7 H2O 7.00 g 6.924 28.091
Na2S2O3 x 5 H2O 2.00 g 1.978 7.971
CaCl2 x 2 H2O 0.40 g 0.396 2.691
NH4Cl 0.20 g 0.198 3.698
KH2PO4 0.25 g 0.247 1.817
Trace minerals 10.00 ml - -
Selenite-tungstate solution 1.00 ml - -
MES 1.95 g 1.929 9.879
Distilled water 1000.00 ml - -
1 Adjust pH to 5.5 with NaOH. Distribute the medium into culture vessels (e.g., 8 ml of the medium into the Balch tubes) under a stream of a H2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, replace the gas phase with H2-CO2-O2 (79:15:6, v/v) gas mixture. Pressurize the inoculated culture vessels to 170 kPa with the same gas mixture.

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 5.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 6.954 28.212
Na2S2O3 x 5 H2O 1.978 7.971
MES 1.929 9.879
MgCl2 x 6 H2O 1.345 6.617
KCl 0.495 6.634
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
phyla 1 1 strain
oxygen microaerophile 1 strain
temperature thermophilic 1 strain