Name: CLOSTRIDIISALIBACTER PAUCIVORANS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J573 #

Equipment needed: Autoclave Hungate tubes Anaerobic workbench Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.3	g	0.329	2.42
K₂HPO₄	0.3	g	0.329	1.891
NH₄Cl	1.0	g	1.098	20.522
NaCl	40.0	g	43.908	751.331
KCl	0.1	g	0.11	1.472
CaCl₂	0.1	g	0.11	0.989
Yeast extract	2.0	g	2.195	-
Trace minerals	10.0	ml	-	-
Resazurin	1.0	mg	0.001	0.005
Distilled water	900.0	ml	-	-
1 To complete the medium, add the following stock solutions [Filter-sterilize NaHCO₃ solution and store under a N₂-CO₂ (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N₂ atmosphere] to the medium (per 4.5 ml):
MgCl₂ x 6 H₂O (15%)	0.1	ml	-	-
NaHCO₃ (5%)	0.3	ml	-	-
Na₂S x 9 H₂O (2%)	0.1	ml	-	-
2 Mix ingredients and adjust pH to 7.0. Distribute 0.9 volume of the medium into tubes (e.g., 4.5 ml of the medium in the Hungate tubes) under a N₂-CO₂ (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave.

Trace minerals #

1000

Solution occurs in 97 other media (first defined in Medium J151).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MgSO₄ x 7 H₂O	3.00	g	3	12.172
MnSO₄ x n H₂O	0.50	g	0.5	-
NaCl	1.00	g	1	17.112
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
CoSO₄ x 7 H₂O	0.10	g	0.1	0.645
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂	0.01	g	0.01	0.039
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
Distilled water	1000.00	ml	-	-
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	7.0
Equipment needed:	Anaerobic workbench, Autoclave, Filter, Hungate tubes

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