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JCM 574 . DB CHARACTERIZATION MEDIUM NO. 2

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J574 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
MDS salt water 833.00 ml - -
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Peptone
(Oxoid) 		0.25 g 0.25 -
Yeast extract
(BD-Difco) 		0.05 g 0.05 -
1 Add components and bring volume to 980 ml with distilled water. For preparation of solid medium, add 20 g/L agar (if necessary, use washed agar as described below). Autoclave, and after cooling, add the following solutions (filter-sterilized or *autoclaved) to the medium:
NH4Cl
(1 M) 		5.00 ml 0.267 5
Potassium phosphate buffer
(*) 		2.00 ml - -
Vitamin solution 3.00 ml - -
Sodium pyruvate
(1 M) 		10.00 ml 1.1 10
2 Readjust pH to 7.0 - 7.5 with a sterilized 10% Na2CO3 solution, if necessary.

Washed agar #

1 Stir agar in distilled water and stand still for 30 min. Then, remove supernatant by decantation. Repeat the washing at least twice.

MDS salt water #

1000
Solution occurs in 5 other media (first defined in Medium J574).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 240 g 240 4106.78
MgCl2 x 6 H2O 30 g 30 147.563
MgSO4 x 7 H2O 35 g 35 142.002
KCl 7 g 7 93.897
CaCl2
(1 M) 		5 ml 0.555 5
1 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.5 with 1 M Tris base.

Potassium phosphate buffer #

100
Solution occurs in 2 other media (first defined in Medium J574).
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4
(1 M) 		83.4 ml 14.526 83.4
KH2PO4
(1 M) 		16.6 ml 2.259 16.6
1 Combine the two solutions and check that pH is close to 7.5. Add an equal volume of distilled water.

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 3 mg 0.003 0.012
Folic acid 4 mg 0.004 0.009
Pyridoxine hydrochloride 50 mg 0.05 0.243
Thiamine HCl 33 mg 0.033 0.098
Riboflavin 10 mg 0.01 0.027
Nicotinic acid 33 mg 0.033 0.268
DL-Calcium pantothenate 17 mg 0.017 0.036
Vitamin B12 17 mg 0.017 0.013
p-Aminobenzoic acid 13 mg 0.013 0.095
Lipoic acid 10 mg 0.01 0.048
Distilled water 1000 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 94

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 199.92 3420.95
MgSO4 x 7 H2O 29.155 118.288
MgCl2 x 6 H2O 24.99 122.92
KCl 5.831 78.216
Sodium pyruvate 1.1 10
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Cultivation metadata from 94 strains BacDive

halophily halophilic 3 strains
halophily extremely halophilic 3 strains
pH 6 - 8 23 strains
pH 8 - 10 19 strains
pH 4 - 6 12 strains
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