Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MDS salt water | 833.00 | ml | - | - | |
FeCl2 solution | 1.00 | ml | - | - | |
Trace element solution | 1.00 | ml | - | - | |
Peptone
(Oxoid) |
0.25 | g | 0.25 | - | |
Yeast extract
(BD-Difco) |
0.05 | g | 0.05 | - | |
1 Add components and bring volume to 980 ml with distilled water. For preparation of solid medium, add 20 g/L agar (if necessary, use washed agar as described below). Autoclave, and after cooling, add the following solutions (filter-sterilized or *autoclaved) to the medium: | |||||
NH4Cl
(1 M) |
5.00 | ml | 0.267 | 5 | |
Potassium phosphate buffer
(*) |
2.00 | ml | - | - | |
Vitamin solution | 3.00 | ml | - | - | |
Sodium pyruvate
(1 M) |
10.00 | ml | 1.1 | 10 | |
2 Readjust pH to 7.0 - 7.5 with a sterilized 10% Na2CO3 solution, if necessary. |
1 Stir agar in distilled water and stand still for 30 min. Then, remove supernatant by decantation. Repeat the washing at least twice. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 240 | g | 240 | 4106.78 | |
MgCl2 x 6 H2O | 30 | g | 30 | 147.563 | |
MgSO4 x 7 H2O | 35 | g | 35 | 142.002 | |
KCl | 7 | g | 7 | 93.897 | |
CaCl2
(1 M) |
5 | ml | 0.555 | 5 | |
1 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.5 with 1 M Tris base. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 3 | mg | 0.003 | 0.012 |
Folic acid | 4 | mg | 0.004 | 0.009 |
Pyridoxine hydrochloride | 50 | mg | 0.05 | 0.243 |
Thiamine HCl | 33 | mg | 0.033 | 0.098 |
Riboflavin | 10 | mg | 0.01 | 0.027 |
Nicotinic acid | 33 | mg | 0.033 | 0.268 |
DL-Calcium pantothenate | 17 | mg | 0.017 | 0.036 |
Vitamin B12 | 17 | mg | 0.017 | 0.013 |
p-Aminobenzoic acid | 13 | mg | 0.013 | 0.095 |
Lipoic acid | 10 | mg | 0.01 | 0.048 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | 7.5 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 94 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 199.92 | 3420.95 |
MgSO4 x 7 H2O | 29.155 | 118.288 |
MgCl2 x 6 H2O | 24.99 | 122.92 |
KCl | 5.831 | 78.216 |
Sodium pyruvate | 1.1 | 10 |
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halophily | halophilic | 3 strains |
halophily | extremely halophilic | 3 strains |
pH | 6 - 8 | 23 strains |
pH | 8 - 10 | 19 strains |
pH | 4 - 6 | 12 strains |
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