Main sol. J576 #

Solution A #

1005

Equipment needed: Autoclave Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na₂SO₄	3.00	g	2.985	21.016
KH₂PO₄	0.20	g	0.199	1.462
NH₄Cl	0.30	g	0.299	5.581
NaCl	7.00	g	6.965	119.185
KCl	0.50	g	0.498	6.674
CaCl₂ x 2 H₂O	0.15	g	0.149	1.015
Yeast extract (BD-Difco)	0.10	g	0.1	-
Trace element solution SL12 (see below)	1.00	ml	-	-
Selenite-tungstate solution	1.00	ml	-	-
HEPES	2.38	g	2.368	9.937
Resazurin	1.00	mg	1.0e-3	0.004
Distilled water	960.00	ml	-	-
1 Mix ingredients, adjust pH to 7.5 and autoclave under a N₂ atmosphere. After cooling, add the following stock solutions (filter-sterilized or *autoclaved) to the solution:
MgCl₂ x 6 H₂O (*, 15%)	10.00	ml	-	-
NaHCO₃ (8%)	30.00	ml	-	-
Vitamin solution	1.00	ml	-	-
Thiamine solution	1.00	ml	-	-
Vitamin B12 solution 0.05 g/L	1.00	ml	-	-

Substrate #

Equipment needed: Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Sodium palmitate	2.8	g	-	-
1 Autoclave in culture vessels.
2 To complete the medium, combine Solution A and substrate in the culture vessels under a N₂-CO₂ (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Finally, reduce the medium with 0.01 volume of 5% Na₂S x 9H2O solution (autoclaved and stored under a N₂ atmosphere).

Trace metal solution SL12 #

1000

Solution occurs in 1 other media (first defined in Medium J576).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na₂-EDTA	3.0	g	3	-
FeSO₄ x 7 H₂O	1.1	g	1.1	3.957
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
MnCl₂ x 4 H₂O	50.0	mg	0.05	-
ZnCl₂	42.0	mg	0.042	0.308
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
Na₂MoO₄ x 2 H₂O	18.0	mg	0.018	0.074
H₃BO₃	300.0	mg	0.3	4.851
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
Distilled water	1000.0	ml	-	-

Selenite-tungstate solution #

1000

Solution occurs in 71 other media (first defined in Medium J431).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaOH	0.4	g	0.4	10.001
Na₂SeO₃ x 5 H₂O	6.0	mg	0.006	0.023
Na₂WO₄ x 2 H₂O	8.0	mg	0.008	0.024
Distilled water	1000.0	ml	-	-

Vitamin solution #

100

Solution occurs in 14 other media (first defined in Medium J403).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
p-Aminobenzoic acid	4	mg	0.04	0.292
Biotin	1	mg	0.01	0.041
Nicotinic acid	10	mg	0.1	0.812
DL-Calcium pantothenate	5	mg	0.05	0.105
Pyridoxine hydrochloride	10	mg	0.1	-
Sodium phosphate buffer (10 mM, pH 7.1)	100	ml	-	-

Thiamine solution #

100

Solution occurs in 10 other media (first defined in Medium J403).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Thiamine HCl	10	mg	0.1	-
Sodium phosphate buffer (25 mM, pH 3.4)	100	ml	-	-

Vitamin B12 solution 0.05 g/L #

100

Solution occurs in 18 other media (first defined in Medium J403).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Vitamin B₁₂	5	mg	0.05	0.037
Distilled water	100	ml	-	-

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	7.5
Equipment needed:	Autoclave, Filter

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pH	6 - 8	1 strain
pH	8 - 10	1 strain
phyla	1	1 strain
oxygen	anaerobe	1 strain
temperature	mesophilic	1 strain

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J576
576 . DESULFATIBACILLUS OLEFINIVORANS MEDIUM

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Culture medium recipe