Loading ...

J576. DESULFATIBACILLUS OLEFINIVORANS MEDIUM

Culture medium recipe

Vol.
×

Solution A #

1005
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.00 g 2.985 21.015
2 0.20 g 0.199 1.462
3 0.30 g 0.299 5.581
4 7.00 g 6.965 119.18
5 0.50 g 0.498 6.673
6 0.15 g 0.149 1.015
7
(BD-Difco)
0.10 g 0.1 -
8
(see below)
1.00 ml - -
9 Selenite-tungstate solution 1.00 ml - -
10 2.38 g 2.368 -
11 1.00 mg 1.0e-3 0.004
12 960.00 ml 953.511 52927.9
13
(*, 15%)
10.00 ml - -
14
(8%)
30.00 ml 5.156 61.374
15 Vitamin solution 1.00 ml - -
16 Thiamine solution 1.00 ml - -
17 Vitamin B12 solution 1.00 ml - -
Instructions:
  1. Mix ingredients, adjust pH to 7.5 and autoclave under a N2 atmosphere. After cooling, add the following stock solutions (filter-sterilized or *autoclaved) to the solution: #13-17
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.8 g - -
Instructions:
  1. Autoclave in culture vessels.
  2. To complete the medium, combine Solution A and substrate in the culture vessels under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Finally, reduce the medium with 0.01 volume of 5% Na2S x 9H2O solution (autoclaved and stored under a N2 atmosphere).
Solution occurs in 1 other media (first defined in medium J576 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.0 g 3 -
2 1.1 g 1.1 3.957
3 190.0 mg 0.19 -
4 50.0 mg 0.05 -
5 42.0 mg 0.042 0.308
6 24.0 mg 0.024 -
7 18.0 mg 0.018 0.074
8 300.0 mg 0.3 4.852
9 2.0 mg 0.002 -
10 1000.0 ml 998.207 55408.8
Solution occurs in 14 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4 mg 0.04 0.292
2 1 mg 0.01 0.041
3 10 mg 0.1 0.812
4 5 mg 0.05 -
5 10 mg 0.1 -
6
(10 mM, pH 7.1)
100 ml - -
Solution occurs in 69 other media (first defined in medium J431 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 0.4 10.001
2 6.0 mg 0.006 -
3 8.0 mg 0.008 -
4 1000.0 ml 998.207 55408.8
Solution occurs in 10 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10 mg 0.1 -
2
(25 mM, pH 3.4)
100 ml - -
Solution occurs in 18 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 mg 0.05 0.032
2 100 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Latest comments

No comments for this medium.