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JCM 576 . DESULFATIBACILLUS OLEFINIVORANS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
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Medium for Desulfatiferula olefinivorans DSM 18843

This strain uses the standard medium without modifications.

Solution A #

1005
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SO4 3.00 g 2.985 21.016
KH2PO4 0.20 g 0.199 1.462
NH4Cl 0.30 g 0.299 5.581
NaCl 7.00 g 6.965 119.185
KCl 0.50 g 0.498 6.674
CaCl2 x 2 H2O 0.15 g 0.149 1.015
Yeast extract
(BD-Difco) 		0.10 g 0.1 -
Trace element solution SL12
(see below) 		1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
HEPES 2.38 g 2.368 9.937
Resazurin 1.00 mg 1.0e-3 0.004
Distilled water 960.00 ml - -
1 Mix ingredients, adjust pH to 7.5 and autoclave under a N2 atmosphere. After cooling, add the following stock solutions (filter-sterilized or *autoclaved) to the solution:
MgCl2 x 6 H2O
(*, 15%) 		10.00 ml - -
NaHCO3
(8%) 		30.00 ml - -
Vitamin solution 1.00 ml - -
Thiamine solution 1.00 ml - -
Vitamin B12 solution 0.05 g/L 1.00 ml - -

Substrate #

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sodium palmitate 2.8 g - -
1 Autoclave in culture vessels.
2 To complete the medium, combine Solution A and substrate in the culture vessels under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Finally, reduce the medium with 0.01 volume of 5% Na2S x 9H2O solution (autoclaved and stored under a N2 atmosphere).

Trace metal solution SL12 #

1000
Solution occurs in 1 other media (first defined in Medium J576).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 3.0 g 3 -
FeSO4 x 7 H2O 1.1 g 1.1 3.957
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
MnCl2 x 4 H2O 50.0 mg 0.05 -
ZnCl2 42.0 mg 0.042 0.308
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 18.0 mg 0.018 0.074
H3BO3 300.0 mg 0.3 4.851
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
Distilled water 1000.0 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Vitamin solution #

100
Solution occurs in 14 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
p-Aminobenzoic acid 4 mg 0.04 0.292
Biotin 1 mg 0.01 0.041
Nicotinic acid 10 mg 0.1 0.812
DL-Calcium pantothenate 5 mg 0.05 0.105
Pyridoxine hydrochloride 10 mg 0.1 -
Sodium phosphate buffer
(10 mM, pH 7.1) 		100 ml - -

Thiamine solution #

100
Solution occurs in 10 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine HCl 10 mg 0.1 -
Sodium phosphate buffer
(25 mM, pH 3.4) 		100 ml - -

Vitamin B12 solution 0.05 g/L #

100
Solution occurs in 18 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 5 mg 0.05 0.037
Distilled water 100 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1
Desulfatiferula olefinivorans DSM 18843

No modifications necessary for this strain.

Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain