Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
1 Use Medium No. 284 supplemented with 2.8 g Na2SO4and 0.2 g (final) yeast extract in Solution A (per 900 ml), and with 1.72 g crotonic acid and 0.43 g sodium benzoate in Solution B (per 100 ml) instead of sodium pyruvate. Prior to inoculation, aseptically and anaerobically add 0.01 volume of 5% Na2S x 9H2O solution (autoclaved and stored under a N2 atmosphere) to the medium. Pressurize the inoculated bottles to 170 kPa with N2-CO2 (80:20, v/v). |
Last modified: | 29.10.21 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Autoclave |
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All strains for this medium: | 1 |