Name: BACILLUS AA MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Main sol. J579 #

Solution A #

1011

Equipment needed: Autoclave Hungate tubes

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.3	g	0.297	2.181
K₂HPO₄	0.3	g	0.297	1.704
NH₄Cl	1.0	g	0.989	18.492
NaCl	1.0	g	0.989	16.925
KCl	0.1	g	0.099	1.327
Yeast extract	1.0	g	0.989	-
Trace minerals	10.0	ml	-	-
Distilled water	1000.0	ml	-	-
1 To complete medium, aseptically add the following solutions to the medium (per 10 ml):
Solution B	0.1	ml	-	-
Sodium acetate (filter-sterilized, 1.0 M)	0.2	ml	-	-
NaHCO₃ (filter-sterilized, 5%)	0.4	ml	-	-
2 Mix ingredients and adjust pH to 7.2. Distribute the solution into tubes (e.g., 10 ml in the Hungate tubes) under a N₂-CO₂ gas mixture (4:1, v/v), seal with butyl rubber stoppers and autoclave.

10

Equipment needed: Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
CaCl₂	0.1	g	10	90.106
MgCl₂ x 6 H₂O	0.5	g	50	245.938
Distilled water	10.0	ml	-	-
1 Autoclave under a N₂ atmosphere.

1000

Solution occurs in 97 other media (first defined in Medium J151).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MgSO₄ x 7 H₂O	3.00	g	3	12.172
MnSO₄ x n H₂O	0.50	g	0.5	-
NaCl	1.00	g	1	17.112
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
CoSO₄ x 7 H₂O	0.10	g	0.1	0.645
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂	0.01	g	0.01	0.039
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
Distilled water	1000.00	ml	-	-
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.