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J600. G5G6 MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.50 g 0.567 -
2 0.65 g 0.737 5.415
3 FeCl2 solution 1.00 ml - -
4 Trace element solution 1.00 ml - -
5 0.50 mg 5.7e-4 0.002
6 880.00 ml 995.943 55283.2
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.11 g 2.2 14.965
2 0.10 g 2 9.838
3 50.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.440 g 3.964 50.141
2 3.730 g 33.604 400.011
3 0.175 g 1.577 8.976
4 50.000 ml 449.643 24958.9
5
(1.0 M)
10.000 ml 203.333 2392.31
6 Trace vitamins
(*)
10.000 ml - -
7 Vitamin B12 solution
(*)
1.000 ml - -
8
(water-saturated, 5.0 mM)
40.000 ml 313.514 -
Instructions:
  1. Prepare all solutions and autoclave under a N2 atmosphere. After cooling, combine the solutions and replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Then, add the following autoclaved or *filter-sterilized solutions to the medium: #5-7
  2. Check the final pH to be around 7.3.
  3. Distribute appropriate amount of the medium into culture vessels under the same gas mixture (e.g., 40.0 ml in 120 ml serum bottle) and seal with Viton rubber stoppers. Finally add per liter the following solution from an anaerobic stock (autoclaved): #8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace element solution 1000 ml - -
2 10 mg 0.01 -
Instructions:
  1. Comment: For strain JCM 14632, incubate with a 5-10% inoculum. When toluene is depleted, re-add the toluene solution (normally every 3 to 4 days, until enough cell growth is seen).
Solution occurs in 89 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 70 mg 0.07 0.514
2 100 mg 0.1 -
3 6 mg 0.006 0.097
4 190 mg 0.19 -
5 2 mg 0.002 -
6 24 mg 0.024 -
7 36 mg 0.036 0.149
8 1000 ml 998.207 55408.8
Solution occurs in 87 other media (first defined in medium J187 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(7.7 M, 25%)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 990.0 ml 988.225 54854.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 18 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 mg 0.05 0.032
2 100 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

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