Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Na2HPO4 x 12 H2O | 0.50 | g | 0.567 | 3.993 |
KH2PO4 | 0.65 | g | 0.737 | 5.415 |
FeCl2 solution | 1.00 | ml | - | - |
Trace element solution | 1.00 | ml | - | - |
Resazurin | 0.50 | mg | 5.7e-4 | 0.002 |
Distilled water | 880.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
CaCl2 x 2 H2O | 0.11 | g | 2.2 | 14.965 |
MgCl2 x 6 H2O | 0.10 | g | 2 | 9.838 |
Distilled water | 50.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4HCO3 | 0.440 | g | 3.964 | - | |
NaHCO3 | 3.730 | g | 33.604 | 400.01 | |
L-Cysteine HCl x H2O | 0.175 | g | 1.577 | - | |
Distilled water | 50.000 | ml | - | - | |
1 Prepare all solutions and autoclave under a N2 atmosphere. After cooling, combine the solutions and replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Then, add the following autoclaved or *filter-sterilized solutions to the medium: | |||||
NaNO3
(1.0 M) |
10.000 | ml | - | - | |
Trace vitamins
(*) |
10.000 | ml | - | - | |
Vitamin B12 solution 0.05 g/L
(*) |
1.000 | ml | - | - | |
2 Distribute appropriate amount of the medium into culture vessels under the same gas mixture (e.g., 40.0 ml in 120 ml serum bottle) and seal with Viton rubber stoppers. Finally add per liter the following solution from an anaerobic stock (autoclaved): | |||||
Toluene
(water-saturated, 5.0 mM) |
40.000 | ml | - | - | |
3 Check the final pH to be around 7.3. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Trace element solution | 1000 | ml | - | - | |
AlCl3 x 6 H2O | 10 | mg | 0.01 | 0.041 | |
1 Comment: For strain JCM 14632, incubate with a 5-10% inoculum. When toluene is depleted, re-add the toluene solution (normally every 3 to 4 days, until enough cell growth is seen). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Vitamin B12 | 5 | mg | 0.05 | 0.037 |
Distilled water | 100 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 7.3 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
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