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JCM 600 . G5G6 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Solution A #

882
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 12 H2O 0.50 g 0.567 3.993
KH2PO4 0.65 g 0.737 5.415
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Resazurin 0.50 mg 5.7e-4 0.002
Distilled water 880.00 ml - -

Solution B #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.11 g 2.2 14.965
MgCl2 x 6 H2O 0.10 g 2 9.838
Distilled water 50.00 ml - -

Solution C #

111
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4HCO3 0.440 g 3.964 -
NaHCO3 3.730 g 33.604 400.01
L-Cysteine HCl x H2O 0.175 g 1.577 -
Distilled water 50.000 ml - -
1 Prepare all solutions and autoclave under a N2 atmosphere. After cooling, combine the solutions and replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Then, add the following autoclaved or *filter-sterilized solutions to the medium:
NaNO3
(1.0 M) 		10.000 ml - -
Trace vitamins
(*) 		10.000 ml - -
Vitamin B12 solution 0.05 g/L
(*) 		1.000 ml - -
2 Distribute appropriate amount of the medium into culture vessels under the same gas mixture (e.g., 40.0 ml in 120 ml serum bottle) and seal with Viton rubber stoppers. Finally add per liter the following solution from an anaerobic stock (autoclaved):
Toluene
(water-saturated, 5.0 mM) 		40.000 ml - -
3 Check the final pH to be around 7.3.

Trace element solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace element solution 1000 ml - -
AlCl3 x 6 H2O 10 mg 0.01 0.041
1 Comment: For strain JCM 14632, incubate with a 5-10% inoculum. When toluene is depleted, re-add the toluene solution (normally every 3 to 4 days, until enough cell growth is seen).

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Vitamin B12 solution 0.05 g/L #

100
Solution occurs in 18 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 5 mg 0.05 0.037
Distilled water 100 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.3
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: No strains found

Cultivation metadata from 0 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain