MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 601 . ANCALOMICROBIUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J601 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.250 g 0.248 1.873
Glucose 0.250 g 0.248 1.374
Vitamin solution 10.000 ml - -
Modified Hutner's basal salts 20.000 ml - -
Na2HPO4 0.071 g 0.07 0.495
Distilled water 980.000 ml - -
1 Adjust pH to 7.0.
2 Separately sterilize Vitamin solution by filtration and aseptically add to the medium.

Vitamin solution #

1000
Solution occurs in 1 other media (first defined in Medium J979).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
Biotin 2.0 mg 0.002 0.008
Thiamine-HCl x 2 H2O 5.0 mg 0.005 -
Calcium pantothenate 5.0 mg 0.005 0.01
Folic acid 2.0 mg 0.002 0.005
Riboflavin 5.0 mg 0.005 0.013
Nicotinamide 5.0 mg 0.005 0.041
Distilled water 1000.0 ml - -
1 Filter-sterilize.

Modified Hutner's basal salts #

1000
Solution occurs in 3 other media (first defined in Medium J900).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 -
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
Metals ''44'' 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 7.0 with KOH (about 7.3 g). Then proceed to add salts. Readjust pH to 6.8 with NaOH or H2SO4.

Metals ''44'' #

1000
Solution occurs in 7 other media (first defined in Medium J149).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 250.0 mg 0.25 -
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x n H2O 154.0 mg 0.154 -
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Filter
Edit in Medium builder

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 0.588 2.386
(NH4)2SO4 0.248 1.873
Glucose 0.248 1.374
Nitrilotriacetic acid 0.198 1.036
Na2HPO4 0.07 0.495
Show more
CSV
JSON

Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
temperature mesophilic 2 strains