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J621. HYL MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4.76 g 4.667 -
2 20.00 g 19.608 335.507
3 4.00 g 3.922 27.608
4 4.30 g 4.216 20.736
5 0.25 g 0.245 4.582
6 0.34 g 0.333 4.471
7 0.14 g 0.137 0.934
8 0.14 g 0.137 0.788
9 1.00 g 0.98 -
10
(50%)
4.50 ml - -
11 1.00 mg 9.8e-4 0.004
12 1000.00 ml 978.634 54322.4
13 Trace elements 1.00 ml - -
14
(in 0.037% HCl, 0.2%)
1.00 ml - -
15 Trace vitamins 10.00 ml - -
16
(0.5 M)
4.00 ml - -
Instructions:
  1. Adjust pH to 7.0.
  2. Autoclave and cool down to room temperature while flushing with N2. Then add the following solutions from sterile anaerobic stocks: #13-16
Solution occurs in 2 other media (first defined in medium J621 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 29.00 g 29 -
2 3.26 g 3.26 -
3 1.80 g 1.8 -
4 1.00 g 1 -
5 0.11 g 0.11 -
6 0.10 g 0.1 -
7 0.10 g 0.1 1.617
8 0.10 g 0.1 -
9 0.10 g 0.1 0.413
10 0.10 g 0.1 -
11 0.05 g 0.05 0.289
12 1000.00 ml 998.207 55408.8
Instructions:
  1. First dissolve EDTA x 2Na and adjust pH to 8.0 with KOH. Then add the minerals.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 989.399 54919.9
NaCl 19.608 335.507
HEPES 4.667 -
MgCl2 x 6H2O 4.216 20.736
Na2SO4 3.922 27.608
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