J621
621 .
HYL MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J621 #
ml
Equipment needed: Anaerobic workbench
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| HEPES | 4.76 | g | 4.667 | 19.582 | |
| NaCl | 20.00 | g | 19.608 | 335.521 | |
| Na2SO4 | 4.00 | g | 3.922 | 27.609 | |
| MgCl2 x 6 H2O | 4.30 | g | 4.216 | 20.736 | |
| NH4Cl | 0.25 | g | 0.245 | 4.582 | |
| KCl | 0.34 | g | 0.333 | 4.471 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.137 | 0.934 | |
| K2HPO4 | 0.14 | g | 0.137 | 0.788 | |
| Yeast extract | 1.00 | g | 0.98 | - | |
|
Sodium lactate
(50%) |
4.50 | ml | - | - | |
| Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Autoclave and cool down to room temperature while flushing with N2. Then add the following solutions from sterile anaerobic stocks: | |||||
| Trace elements | 1.00 | ml | - | - | |
|
Fe(NH4)2(SO4)2 x 6 H2O
(in 0.037% HCl, 0.2%) |
1.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
|
Na2S x 9 H2O
(0.5 M) |
4.00 | ml | - | - | |
| 2 Adjust pH to 7.0. | |||||
Solution occurs in 2
other media (first defined in Medium J621).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2-EDTA | 29.00 | g | 29 | - | |
| MnSO4 x n H2O | 3.26 | g | 3.26 | - | |
| CoCl2 x 6 H2O | 1.80 | g | 1.8 | 13.863 | |
| ZnSO4 x 7 H2O | 1.00 | g | 1 | 3.478 | |
| NiSO4 x 6 H2O | 0.11 | g | 0.11 | 0.711 | |
| CuSO4 x 5 H2O | 0.10 | g | 0.1 | 0.401 | |
| H3BO3 | 0.10 | g | 0.1 | 1.617 | |
| AlK(SO4)2 x 12 H2O | 0.10 | g | 0.1 | - | |
| Na2MoO4 x 2 H2O | 0.10 | g | 0.1 | 0.413 | |
| Na2WO4 x 2 H2O | 0.10 | g | 0.1 | 0.303 | |
| Na2SeO3 | 0.05 | g | 0.05 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve EDTA x 2Na and adjust pH to 8.0 with KOH. Then add the minerals. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 8.0 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 19.608 | 335.521 |
| HEPES | 4.667 | 19.582 |
| MgCl2 x 6 H2O | 4.216 | 20.736 |
| Na2SO4 | 3.922 | 27.609 |
| Yeast extract | 0.98 | - |
| Show more | ||
