JCM 621 . HYL MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
HEPES 4.76 g 4.667 19.582
NaCl 20.00 g 19.608 335.521
Na2SO4 4.00 g 3.922 27.609
MgCl2 x 6 H2O 4.30 g 4.216 20.736
NH4Cl 0.25 g 0.245 4.582
KCl 0.34 g 0.333 4.471
CaCl2 x 2 H2O 0.14 g 0.137 0.934
K2HPO4 0.14 g 0.137 0.788
Yeast extract 1.00 g 0.98 -
Sodium lactate
(50%)
4.50 ml - -
Resazurin 1.00 mg 9.8e-4 0.004
Distilled water 1000.00 ml - -
1 Autoclave and cool down to room temperature while flushing with N2. Then add the following solutions from sterile anaerobic stocks:
Trace elements 1.00 ml - -
Fe(NH4)2(SO4)2 x 6 H2O
(in 0.037% HCl, 0.2%)
1.00 ml - -
Trace vitamins 10.00 ml - -
Na2S x 9 H2O
(0.5 M)
4.00 ml - -
2 Adjust pH to 7.0.
Solution occurs in 2 other media (first defined in Medium J621).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 29.00 g 29 -
MnSO4 x n H2O 3.26 g 3.26 -
CoCl2 x 6 H2O 1.80 g 1.8 13.863
ZnSO4 x 7 H2O 1.00 g 1 3.478
NiSO4 x 6 H2O 0.11 g 0.11 0.711
CuSO4 x 5 H2O 0.10 g 0.1 0.401
H3BO3 0.10 g 0.1 1.617
AlK(SO4)2 x 12 H2O 0.10 g 0.1 -
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
Na2WO4 x 2 H2O 0.10 g 0.1 0.303
Na2SeO3 0.05 g 0.05 -
Distilled water 1000.00 ml - -
1 First dissolve EDTA x 2Na and adjust pH to 8.0 with KOH. Then add the minerals.
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 8.0
Equipment needed: Anaerobic workbench, Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.608 335.521
HEPES 4.667 19.582
MgCl2 x 6 H2O 4.216 20.736
Na2SO4 3.922 27.609
Yeast extract 0.98 -
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain
temperature thermophilic 1 strain