Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
HEPES | 4.76 | g | 4.667 | 19.582 | |
NaCl | 20.00 | g | 19.608 | 335.521 | |
Na2SO4 | 4.00 | g | 3.922 | 27.609 | |
MgCl2 x 6 H2O | 4.30 | g | 4.216 | 20.736 | |
NH4Cl | 0.25 | g | 0.245 | 4.582 | |
KCl | 0.34 | g | 0.333 | 4.471 | |
CaCl2 x 2 H2O | 0.14 | g | 0.137 | 0.934 | |
K2HPO4 | 0.14 | g | 0.137 | 0.788 | |
Yeast extract | 1.00 | g | 0.98 | - | |
Sodium lactate
(50%) |
4.50 | ml | - | - | |
Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
Distilled water | 1000.00 | ml | - | - | |
1 Autoclave and cool down to room temperature while flushing with N2. Then add the following solutions from sterile anaerobic stocks: | |||||
Trace elements | 1.00 | ml | - | - | |
Fe(NH4)2(SO4)2 x 6 H2O
(in 0.037% HCl, 0.2%) |
1.00 | ml | - | - | |
Trace vitamins | 10.00 | ml | - | - | |
Na2S x 9 H2O
(0.5 M) |
4.00 | ml | - | - | |
2 Adjust pH to 7.0. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-EDTA | 29.00 | g | 29 | - | |
MnSO4 x n H2O | 3.26 | g | 3.26 | - | |
CoCl2 x 6 H2O | 1.80 | g | 1.8 | 13.863 | |
ZnSO4 x 7 H2O | 1.00 | g | 1 | 3.478 | |
NiSO4 x 6 H2O | 0.11 | g | 0.11 | 0.711 | |
CuSO4 x 5 H2O | 0.10 | g | 0.1 | 0.401 | |
H3BO3 | 0.10 | g | 0.1 | 1.617 | |
AlK(SO4)2 x 12 H2O | 0.10 | g | 0.1 | - | |
Na2MoO4 x 2 H2O | 0.10 | g | 0.1 | 0.413 | |
Na2WO4 x 2 H2O | 0.10 | g | 0.1 | 0.303 | |
Na2SeO3 | 0.05 | g | 0.05 | - | |
Distilled water | 1000.00 | ml | - | - | |
1 First dissolve EDTA x 2Na and adjust pH to 8.0 with KOH. Then add the minerals. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | 8.0 |
Equipment needed: | Anaerobic workbench, Autoclave |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 19.608 | 335.521 |
HEPES | 4.667 | 19.582 |
MgCl2 x 6 H2O | 4.216 | 20.736 |
Na2SO4 | 3.922 | 27.609 |
Yeast extract | 0.98 | - |
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