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JCM 636 . METHANOLOBUS R15 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Solution A #

860
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.54 g 0.628 4.614
Na2HPO4 1.34 g 1.558 10.976
Yeast extract
(BD-Difco) 		3.00 g 3.488 -
Tryptone
(BD-Difco) 		0.50 g 0.581 8.179
Resazurin 1.00 mg 0.001 0.005
Distilled water 860.00 ml - -

Solution B #

102
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.190 g 1.863 34.824
NaCl 0.190 g 1.863 31.875
CaCl2 x 2 H2O 6.000 mg 0.059 0.4
MgCl2 x 6 H2O 0.063 g 0.618 3.038
FeCl2 solution 1.000 ml - -
Trace element solution 1.000 ml - -
Distilled water 100.000 ml - -

Solution C #

44
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Methanol
(50%) 		3.5 ml - -
1 Autoclave Solutions A and B separately and autoclave Solution C in a closed tube or vial. After cooling, combine the three solutions under a stream of N2-CO2 (80:20, v/v), then add the following solutions (filter-sterilized):
Trace vitamins 10.0 ml - -
NaHCO3
(8%) 		30.0 ml - -
2 Distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Prior to inoculation, reduce the medium with 0.025% L-cysteine x HCl x H2O and 0.025% Na2S x 9H2O (autoclaved as 5% solutions under a N2 atmosphere).

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: No strains found