J641
641 .
NCDC-99X MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J641 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Mineral solution 1 | 37.50 | ml | - | - | |
| Salt Solution II | 37.50 | ml | - | - | |
| Yeast extract | 1.00 | g | 13.333 | - | |
| Resazurin | 1.00 | mg | 0.013 | 0.058 | |
| Arginine | 2.11 | g | 28.133 | 161.5 | |
| Histidine | 1.55 | g | 20.667 | 133.204 | |
|
Phytone peptone
(BD-BBL) |
2.00 | g | 26.667 | - | |
| Na2CO3 | 4.00 | g | 53.333 | 503.202 | |
| L-Cysteine HCl x H2O | 0.50 | g | 6.667 | - | |
| 1 Dissolve components except Na2CO3 and L-cysteine x HCl x H2O in distilled water and bring volume to 1.0 L. Adjust pH to 7.0. Bring to a boil and cool down under a stream of a N2-CO2 (4:1, v/v) gas mixture. Add Na2CO3 and L-cysteine x HCl x H2O to the medium and dispense into culture vessels under the same gas mixture. Seal with butyl rubber stoppers and autoclave. | |||||
Solution occurs in 4
other media (first defined in Medium 203).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| K2HPO4 | 6 | g | 6 | 34.448 |
| Distilled water | 1000 | ml | - | - |
Solution occurs in 1
other media (first defined in Medium 1281).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| KH2PO4 | 6.0 | g | 6 | 44.09 |
| (NH4)2SO4 | 12.0 | g | 12 | 90.813 |
| NaCl | 12.0 | g | 12 | 205.339 |
| MgSO4 x 7 H2O | 1.2 | g | 1.2 | 4.869 |
| CaCl2 x 2 H2O | 1.2 | g | 1.2 | 8.162 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2CO3 | 53.333 | 503.202 |
| Arginine | 28.133 | 161.5 |
| Phytone peptone | 26.667 | - |
| Histidine | 20.667 | 133.204 |
| Yeast extract | 13.333 | - |
| Show more | ||
