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JCM 642 . MOORELLA AN10 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Solution A #

862
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 1.000 g 1.16 -
Casitone
(BD-Difco) 		1.000 g 1.16 -
K2HPO4 0.348 g 0.404 2.318
KH2PO4 0.227 g 0.263 1.935
NH4Cl 0.500 g 0.58 10.844
MgSO4 x 7 H2O 0.500 g 0.58 2.353
CaCl2 x 2 H2O 0.250 g 0.29 1.973
NaCl 2.250 g 2.61 44.665
FeSO4 x 6 H2O 0.100 g 0.116 0.764
Trace vitamins 10.000 ml - -
Trace element solution 1.000 ml - -
Se/W solution 1.000 ml - -
L-Cysteine HCl x H2O 0.500 g 0.58 -
Na2S x 9 H2O 0.500 g 0.58 2.415
Resazurin 1.000 mg 0.001 0.005
Distilled water 850.000 ml - -
1 Mix components and autoclave under a N2-CO2 (4:1, v/v) atmosphere.

Solution B #

100
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fructose 5 g 50 277.531
Distilled water 100 ml - -
1 Autoclave under a N2 atmosphere.

Solution C #

20
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3
(8%) 		20 ml - -
1 Filter-sterilize and store under a N2-CO2 (4:1, v/v) atmosphere.
2 To complete the medium, mix Solutions A, B and C under a N2-CO2 (4:1, v/v) atmosphere. Reduce the medium with 0.3 g/L L-cysteine x HCl x H2O and 0.3 g/L Na2S x 9H2O from sterile stock solutions (autoclaved and stored under a N2 atmosphere).

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace element solution #

Solution occurs in 1 other media (first defined in Medium J480).
1 Use Trace element solution of Medium No. 243 without Na2SeO3 x 5H2O.

Se/W solution #

1000
Solution occurs in 4 other media (first defined in Medium J317).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 4 mg 0.004 -
Na2WO4 x 2 H2O 4 mg 0.004 0.012
Distilled water 1000 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: No strains found