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J642. MOORELLA AN10 MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.000 g 1.16 -
2
(BD-Difco)
1.000 g 1.16 -
3 0.348 g 0.404 2.318
4 0.227 g 0.263 1.935
5 0.500 g 0.58 10.844
6 0.500 g 0.58 2.353
7 0.250 g 0.29 1.973
8 2.250 g 2.61 44.663
9 0.100 g 0.116 0.446
10 Trace vitamins 10.000 ml - -
11 Trace element solution 1.000 ml - -
12 Se/W solution 1.000 ml - -
13 0.500 g 0.58 3.302
14 0.500 g 0.58 2.415
15 1.000 mg 0.001 0.005
16 850.000 ml 984.311 54637.5
Instructions:
  1. Mix components and autoclave under a N2-CO2 (4:1, v/v) atmosphere.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 g 50 -
2 100 ml 998.207 55408.8
Instructions:
  1. Autoclave under a N2 atmosphere.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(8%)
20 ml 172.72 2056.03
Instructions:
  1. Filter-sterilize and store under a N2-CO2 (4:1, v/v) atmosphere.
  2. To complete the medium, mix Solutions A, B and C under a N2-CO2 (4:1, v/v) atmosphere. Reduce the medium with 0.3 g/L L-cysteine x HCl x H2O and 0.3 g/L Na2S x 9H2O from sterile stock solutions (autoclaved and stored under a N2 atmosphere).
Solution occurs in 1 other media (first defined in medium J480 )
Instructions:
  1. Use Trace element solution of Medium No. 243 without Na2SeO3 x 5H2O.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 4 other media (first defined in medium J317 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4 mg 0.004 0.023
2 4 mg 0.004 -
3 1000 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

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