J643
643 .
VRI MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J643 #
ml
Equipment needed: Filter
Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.00 | g | 0.98 | 18.329 | |
| KH2PO4 | 0.40 | g | 0.392 | 2.882 | |
| MgCl2 x 6 H2O | 0.60 | g | 0.588 | 2.893 | |
| NaCl | 46.00 | g | 45.098 | 771.698 | |
| KCl | 1.00 | g | 0.98 | 13.151 | |
| Fe(NH4)2(SO4)2 x 6 H2O | 0.01 | g | 0.01 | - | |
| Yeast extract | 2.00 | g | 1.961 | - | |
|
Trypticase peptone
(BD-BBL) |
2.00 | g | 1.961 | - | |
| Trace element solution | 10.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.49 | - | |
| Na2S x 9 H2O | 0.50 | g | 0.49 | 2.041 | |
| Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix ingredients except Trace vitamins, L-cysteine x HCl x H2O and Na2S x 9H2O. Bring to a boil for 5-10 sec, then, cool down under a stream of H2-CO2 (80:20, v/v). Dispense the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Separately autoclave L-cysteine x HCl x H2O and Na2S x 9H2O as 5% solutions under a N2 atmosphere. Filter-sterilize Trace vitamins solution. After cooling the medium, add Trace vitamins and adjust pH to 9.0 with 40% (w/v) Na2CO3 solution (autoclaved and stored under a N2 atmosphere). Prior to inoculation, aseptically and anaerobically add the L-cysteine x HCl x H2O and Na2S x 9H2O solutions. Pressurize inoculated vessels to 100 kPa H2-CO2 (80:20, v/v). | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2-EDTA x 2 H2O | 0.5 | g | 0.5 | - | |
| CoCl2 x 6 H2O | 0.1 | g | 0.1 | 0.77 | |
| MnCl2 x 4 H2O | 0.1 | g | 0.1 | - | |
| FeSO4 x 6 H2O | 0.1 | g | 0.1 | 0.658 | |
| ZnCl2 | 0.1 | g | 0.1 | 0.734 | |
| AlCl3 x 6 H2O | 0.4 | g | 0.4 | 1.657 | |
| Na2WO4 x 2 H2O | 0.3 | g | 0.3 | 0.91 | |
| CuCl2 x 2 H2O | 0.2 | g | 0.2 | 1.173 | |
| NiSO4 x 6 H2O | 0.2 | g | 0.2 | 1.292 | |
| Na2SeO3 x 5 H2O | 0.1 | g | 0.1 | 0.38 | |
| H3BO3 | 0.1 | g | 0.1 | 1.617 | |
| Na2MoO4 x 2 H2O | 0.1 | g | 0.1 | 0.413 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve EDTA x 2Na x 2H2O first in 500 ml distilled water and adjust pH to 6.5 with KOH. Then add the remaining salts and adjust the volume to 1.0 L. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 6.5 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |