Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
1 Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): | |||||
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 1.00 | g | 1 | 17.112 | |
KCl | 0.50 | g | 0.5 | 6.707 | |
MgCl2 x 6 H2O | 0.40 | g | 0.4 | 1.968 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
NH4Cl | 0.30 | g | 0.3 | 5.609 | |
KH2PO4 | 0.20 | g | 0.2 | 1.47 | |
Na2SO4 | 0.15 | g | 0.15 | 1.056 | |
Resazurin | 0.40 | mg | 4.0e-4 | 0.002 | |
Distilled water | 910.00 | ml | - | - | |
NaHCO3
(*, 8%) |
40.00 | ml | - | - | |
Trace element solution | 1.00 | ml | - | - | |
FeCl2 solution | 1.00 | ml | - | - | |
Selenite-tungstate solution | 1.00 | ml | - | - | |
Trace vitamins (see Medium No. 197)
(*) |
10.00 | ml | - | - | |
Vitamin B12 solution (see Medium No. 403)
(*) |
1.00 | ml | - | - | |
Fatty acid mixture (see Medium No. 266)
(*) |
1.00 | ml | - | - | |
Yeast extract
(10%) |
10.00 | ml | - | - | |
Casamino acids
(10%) |
10.00 | ml | - | - | |
Glucose
(1 M) |
5.00 | ml | - | - | |
Dithiothreitol
(*, 0.1 M) |
10.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnCl2 | 70 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaOH | 0.4 | g | 0.4 | 10.001 |
Na2SeO3 x 5 H2O | 6.0 | mg | 0.006 | 0.023 |
Na2WO4 x 2 H2O | 8.0 | mg | 0.008 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 29.10.21 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 2 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 1 | 17.112 |
KCl | 0.5 | 6.707 |
MgCl2 x 6 H2O | 0.4 | 1.968 |
NH4Cl | 0.3 | 5.609 |
KH2PO4 | 0.2 | 1.47 |
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