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JCM 670 . SPIROCHAETA CALDARIA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J670 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 2.00 g 1.896 10.523
Tryptone
(BD-Difco) 		2.00 g 1.896 26.67
L-Cysteine HCl x H2O 0.25 g 0.237 -
Resazurin 0.50 mg 4.7e-4 0.002
Distilled water 1000.00 ml - -
1 Mix ingredients and distribute into culture vessels under a N2 atmosphere, seal with butyl rubber stoppers, and autoclave. Separately autoclave or *filter-sterilize the following solutions under a N2 atmosphere and add to the medium (per liter):
FeCl2 solution 10.00 ml - -
Trace element solution 10.00 ml - -
Trace minerals 25.00 ml - -
Trace vitamins (see Medium No. 197)
(*) 		10.00 ml - -
2 Adjust pH of the medium to 7.0 with sterile NaOH, if necessary.

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Last modified: 03.04.22
Source: JCM
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tryptone 1.896 26.67
Glucose 1.896 10.523
L-Cysteine HCl x H2O 0.237 -
MgSO4 x 7 H2O 0.071 0.288
Nitrilotriacetic acid 0.036 0.186
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