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JCM 671 . THERMOANAEROBIUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 3.00 g 2.93 -
Tryptone
(BD-Difco)
10.00 g 9.766 137.389
NH4Cl 0.90 g 0.879 16.431
NaCl 0.90 g 0.879 15.04
MgCl2 x 6 H2O 0.40 g 0.391 1.921
KH2PO4 0.75 g 0.732 5.382
K2HPO4 1.50 g 1.465 8.41
Trace element solution 9.00 ml - -
Trace vitamins 5.00 ml - -
FeSO4 x 7 H2O 3.00 mg 0.003 0.011
Resazurin 1.00 mg 9.8e-4 0.004
Distilled water 950.00 ml - -
1 Mix ingredients and adjust pH to 7.0. Distribute the medium into culture vessels under a N2 atmosphere, seal with butyl rubber stoppers and autoclave. Separately autoclave the following solutions under a N2 atmosphere and add to the medium (per 950 ml):
Glucose
(10%)
50.00 ml - -
Na2S x 9 H2O
(5%)
10.00 ml - -
2 Readjust pH of the medium to 7.2 - 7.4 with sterile NaOH, if necessary.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.800 g 12.8 66.967
FeCl2 x 4 H2O 0.200 g 0.2 1.006
MnCl2 x 4 H2O 0.100 g 0.1 -
CoCl2 x 6 H2O 0.170 g 0.17 1.309
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnCl2 0.100 g 0.1 0.734
CuCl2 x 2 H2O 0.020 g 0.02 0.117
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.026 g 0.026 0.201
NaCl 1.000 g 1 17.112
Na2SeO3 0.020 g 0.02 -
Distilled water 1000.000 ml - -
1 Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH 6.5 with KOH. Then add the remaining salts and adjust volume to 1000 ml.
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3
Equipment needed: Autoclave

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tryptone 9.766 137.389
Yeast extract 2.93 -
K2HPO4 1.465 8.41
NaCl 0.888 15.19
NH4Cl 0.879 16.431
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain

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