Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Yeast extract | 3.00 | g | 2.93 | - | |
Tryptone
(BD-Difco) |
10.00 | g | 9.766 | 137.389 | |
NH4Cl | 0.90 | g | 0.879 | 16.431 | |
NaCl | 0.90 | g | 0.879 | 15.04 | |
MgCl2 x 6 H2O | 0.40 | g | 0.391 | 1.921 | |
KH2PO4 | 0.75 | g | 0.732 | 5.382 | |
K2HPO4 | 1.50 | g | 1.465 | 8.41 | |
Trace element solution | 9.00 | ml | - | - | |
Trace vitamins | 5.00 | ml | - | - | |
FeSO4 x 7 H2O | 3.00 | mg | 0.003 | 0.011 | |
Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
Distilled water | 950.00 | ml | - | - | |
1 Mix ingredients and adjust pH to 7.0. Distribute the medium into culture vessels under a N2 atmosphere, seal with butyl rubber stoppers and autoclave. Separately autoclave the following solutions under a N2 atmosphere and add to the medium (per 950 ml): | |||||
Glucose
(10%) |
50.00 | ml | - | - | |
Na2S x 9 H2O
(5%) |
10.00 | ml | - | - | |
2 Readjust pH of the medium to 7.2 - 7.4 with sterile NaOH, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 12.800 | g | 12.8 | 66.967 | |
FeCl2 x 4 H2O | 0.200 | g | 0.2 | 1.006 | |
MnCl2 x 4 H2O | 0.100 | g | 0.1 | - | |
CoCl2 x 6 H2O | 0.170 | g | 0.17 | 1.309 | |
CaCl2 x 2 H2O | 0.100 | g | 0.1 | 0.68 | |
ZnCl2 | 0.100 | g | 0.1 | 0.734 | |
CuCl2 x 2 H2O | 0.020 | g | 0.02 | 0.117 | |
H3BO3 | 0.010 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.010 | g | 0.01 | 0.041 | |
NiCl2 x 6 H2O | 0.026 | g | 0.026 | 0.201 | |
NaCl | 1.000 | g | 1 | 17.112 | |
Na2SeO3 | 0.020 | g | 0.02 | - | |
Distilled water | 1000.000 | ml | - | - | |
1 Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH 6.5 with KOH. Then add the remaining salts and adjust volume to 1000 ml. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.3 |
Equipment needed: | Autoclave |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Tryptone | 9.766 | 137.389 |
Yeast extract | 2.93 | - |
K2HPO4 | 1.465 | 8.41 |
NaCl | 0.888 | 15.19 |
NH4Cl | 0.879 | 16.431 |
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