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JCM 673 . METHANOPYRUS MEDIUM FOR STRAIN 116

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J673 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 19.608 335.521
K2HPO4 0.07 g 0.069 0.394
KH2PO4 0.09 g 0.088 0.648
CaCl2 x 2 H2O 0.80 g 0.784 5.335
NH4Cl 0.30 g 0.294 5.499
MgSO4 x 7 H2O 6.00 g 5.882 23.866
MgCl2 x 6 H2O 1.00 g 0.98 4.822
KCl 0.33 g 0.324 4.34
NiCl2 x 6 H2O 5.00 mg 0.005 0.038
Na2SeO3 x 5 H2O 5.00 mg 0.005 0.019
Fe2(SO4)3 x n H2O 0.05 g 0.049 -
FeSO4 x 7 H2O 0.01 g 0.01 0.035
Na2WO4 x 2 H2O 5.00 mg 0.005 0.015
Resazurin 1.00 mg 9.8e-4 0.004
Marine trace elements solution 30.00 ml - -
Iron
(powder, 20 - 60 mesh, Nacalai) 		20.00 g 19.608 351.143
NaHCO3 2.00 g 1.961 23.341
Trace vitamins 10.00 ml - -
Trace mineral solution 10.00 ml - -
Na2S x 9 H2O 0.50 g 0.49 2.041
Distilled water 970.00 ml - -
1 Mix components except iron powder and filter-sterilize. Dispense the medium and iron powder (autoclaved) into sterile culture vessels (typically, 15 - 20 ml medium in 120 ml serum bottles) under a H2-CO2 (80:20, v/v) gas atmosphere and seal with butyl rubber stoppers. Prior to inoculation, reduce the medium with 0.01 volume of 5% Na2S x 9H2O solution (neutralized with H2SO4, autoclaved and stored under a N2 atmosphere). Pressurize the inoculated bottle to 200 kPa H2-CO2 (80:20, v/v).

Marine trace elements solution #

1000
Solution occurs in 1 other media (first defined in Medium J216).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaBr 4.00 g 4 38.877
SrCl2 x 6 H2O 1.80 g 1.8 6.751
H3BO3 1.30 g 1.3 21.022
KI 1.25 mg 0.001 0.005
Sodium silicate 100.00 mg 0.1 0.819
NaF 60.00 mg 0.06 1.429
KNO3 40.00 mg 0.04 0.396
Na2HPO4 x 12 H2O 0.46 g 0.46 3.24
Distilled water 1000.00 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace mineral solution #

1000
Solution occurs in 2 other media (first defined in Medium J368).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MnSO4 x n H2O 0.50 g 0.5 -
CoSO4 x 7 H2O 0.50 g 0.5 3.226
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.02 g 0.02 0.077
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
SrCl2 x 6 H2O 0.01 g 0.01 0.038
NaBr 0.01 g 0.01 0.097
KI 0.01 g 0.01 0.039
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Iron 19.608 351.143
NaCl 19.608 335.521
MgSO4 x 7 H2O 5.882 23.866
NaHCO3 1.961 23.341
MgCl2 x 6 H2O 0.98 4.822
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature hyperthermophilic 1 strain