Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 20.00 | g | 19.608 | 335.521 | |
K2HPO4 | 0.07 | g | 0.069 | 0.394 | |
KH2PO4 | 0.09 | g | 0.088 | 0.648 | |
CaCl2 x 2 H2O | 0.80 | g | 0.784 | 5.335 | |
NH4Cl | 0.30 | g | 0.294 | 5.499 | |
MgSO4 x 7 H2O | 6.00 | g | 5.882 | 23.866 | |
MgCl2 x 6 H2O | 1.00 | g | 0.98 | 4.822 | |
KCl | 0.33 | g | 0.324 | 4.34 | |
NiCl2 x 6 H2O | 5.00 | mg | 0.005 | 0.038 | |
Na2SeO3 x 5 H2O | 5.00 | mg | 0.005 | 0.019 | |
Fe2(SO4)3 x n H2O | 0.05 | g | 0.049 | - | |
FeSO4 x 7 H2O | 0.01 | g | 0.01 | 0.035 | |
Na2WO4 x 2 H2O | 5.00 | mg | 0.005 | 0.015 | |
Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
Marine trace elements solution | 30.00 | ml | - | - | |
Iron
(powder, 20 - 60 mesh, Nacalai) |
20.00 | g | 19.608 | 351.143 | |
NaHCO3 | 2.00 | g | 1.961 | 23.341 | |
Trace vitamins | 10.00 | ml | - | - | |
Trace mineral solution | 10.00 | ml | - | - | |
Na2S x 9 H2O | 0.50 | g | 0.49 | 2.041 | |
Distilled water | 970.00 | ml | - | - | |
1 Mix components except iron powder and filter-sterilize. Dispense the medium and iron powder (autoclaved) into sterile culture vessels (typically, 15 - 20 ml medium in 120 ml serum bottles) under a H2-CO2 (80:20, v/v) gas atmosphere and seal with butyl rubber stoppers. Prior to inoculation, reduce the medium with 0.01 volume of 5% Na2S x 9H2O solution (neutralized with H2SO4, autoclaved and stored under a N2 atmosphere). Pressurize the inoculated bottle to 200 kPa H2-CO2 (80:20, v/v). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaBr | 4.00 | g | 4 | 38.877 |
SrCl2 x 6 H2O | 1.80 | g | 1.8 | 6.751 |
H3BO3 | 1.30 | g | 1.3 | 21.022 |
KI | 1.25 | mg | 0.001 | 0.005 |
Sodium silicate | 100.00 | mg | 0.1 | 0.819 |
NaF | 60.00 | mg | 0.06 | 1.429 |
KNO3 | 40.00 | mg | 0.04 | 0.396 |
Na2HPO4 x 12 H2O | 0.46 | g | 0.46 | 3.24 |
Distilled water | 1000.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
CoSO4 x 7 H2O | 0.50 | g | 0.5 | 3.226 | |
ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 | 0.02 | g | 0.02 | 0.077 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
SrCl2 x 6 H2O | 0.01 | g | 0.01 | 0.038 | |
NaBr | 0.01 | g | 0.01 | 0.097 | |
KI | 0.01 | g | 0.01 | 0.039 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0. |
Last modified: | 29.10.21 |
Source: | JCM |
Taxonomic range: | Archaea |
Medium type: | Defined medium |
Final pH: | n.d. |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Iron | 19.608 | 351.143 |
NaCl | 19.608 | 335.521 |
MgSO4 x 7 H2O | 5.882 | 23.866 |
NaHCO3 | 1.961 | 23.341 |
MgCl2 x 6 H2O | 0.98 | 4.822 |
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